Molecular systematics of Goodyerinae (Cranichideae, Orchidoideae, Orchidaceae) based on multiple nuclear and plastid regions

Shi-Pin Chen; Huai-Zhen Tian; Qiu-Xiang Guan; Jun-Wen Zhai; Guo-Qiang Zhang; Li-Jun Chen; Zhong-Jian Liu; Si-Ren Lan; Ming-He Li

doi:10.1016/j.ympev.2019.106542

Molecular systematics of Goodyerinae (Cranichideae, Orchidoideae, Orchidaceae) based on multiple nuclear and plastid regions

Mol Phylogenet Evol. 2019 Oct:139:106542. doi: 10.1016/j.ympev.2019.106542. Epub 2019 Jun 20.

Authors

Shi-Pin Chen¹, Huai-Zhen Tian², Qiu-Xiang Guan¹, Jun-Wen Zhai¹, Guo-Qiang Zhang³, Li-Jun Chen³, Zhong-Jian Liu¹, Si-Ren Lan⁴, Ming-He Li⁵

Affiliations

¹ Key Laboratory of National Forestry and Grassland Administration for Orchid Conservation and Utilization, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
² School of Life Sciences, East China Normal University, Shanghai 200241, China.
³ Shenzhen Key Laboratory for Orchid Conservation and Utilization, The National Orchid Conservation Center of China and The Orchid Conservation & Research Center of Shenzhen, Shenzhen 518114, China.
⁴ Key Laboratory of National Forestry and Grassland Administration for Orchid Conservation and Utilization, Fujian Agriculture and Forestry University, Fuzhou 350002, China. Electronic address: lkxz@fafu.edu.
⁵ Key Laboratory of National Forestry and Grassland Administration for Orchid Conservation and Utilization, Fujian Agriculture and Forestry University, Fuzhou 350002, China. Electronic address: fjalmh@126.com.

PMID: 31229601
DOI: 10.1016/j.ympev.2019.106542

Abstract

Goodyerinae are one of the most species-rich and widespread subtribes of Orchidaceae but notorious for their taxonomic difficulty. Here, a comprehensive molecular phylogenetic study of the subtribe is presented based on two nuclear (ITS, Xdh) and five plastid (matK, psaB, rbcL, trnL, trnL-F) regions. A total of 119 species were included representing all clades recovered by previous phylogenetic analyses as well as seven outgroups. Maximum parsimony, maximum likelihood and Bayesian inference methods were used to infer the phylogenetic relationships. The results show that the Goodyerinae subdivided into three major subdivisions and six groupings: Pachyplectron, Goodyera clade (including Goodyera procera, Microchilus subclade and Goodyera subclade) and Cheirostylis clade (including Gonatostylis, Cheirostylis subclade and Ludisia subclade). Four genera, Erythrodes, Goodyera, Myrmechis and Odontochilus, are not monophyletic. The results support Odontochilus s. l. to include Myrmechis and Kuhlhasseltia. The systematic positions of Goodyera procera and two isolated genera, Herpysma and Orchipedum, are difficult to determine.

Keywords: Cheirostylidinae; Jewel orchid; Ludisiinae; Pachyplectroninae; Physurinae; Terrestrial orchids.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Bayes Theorem
Cell Nucleus / genetics
Orchidaceae / classification*
Orchidaceae / genetics
Phylogeny
Plastids / genetics