In this work, we demonstrate a highly effective method to generate and detect single-nanoparticle (NP) collision events on a nanoelectrode in aqueous solutions. The nanoelectrode of a nanopore-nanoelectrode nanopipette is first employed to accumulate NPs in solution by dielectrophoresis (DEP). Instead of using amperometric methods, the continuous individual NP collision events on the nanoelectrode are sensitively detected by monitoring the open-circuit potential changes of the nanoelectrode. Metallic gold NPs (GNPs) and insulating polystyrene (PS) NPs with various sizes are used as the model NPs. Due to the higher conductivity and polarizability of GNPs, the collision motion of a GNP is different from that of a PS NP. The difference is distinct in the shape of the transient potential change and its first time derivative detected by the nanoelectrode. Therefore, the collision events by metallic and insulating NPs on a nanoelectrode can be differentiated based on their polarizability. DEP induced NP separation and cluster formation can also be probed in detail in the concentrated mixture of PS NPs and GNPs.