Abstract
Mucosal-associated invariant T (MAIT) cells recognize intermediates of the vitamin B2 biosynthetic pathway present in a variety of bacteria, presented by the monomorphic MR1 molecules. Because of their central role in shaping adaptive immunity through interaction with dendritic cells (DCs) and B cells, their manipulation can be of translational relevance. We describe a method to routinely isolate and maintain MAIT cells from peripheral blood and to investigate their activity using DC as targets.
Keywords:
CD40L; MAIT; MR1 dendritic cells.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Antigens, Bacterial / immunology
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Biomarkers
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Cell Communication* / immunology
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Cell Differentiation
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Cell Separation / methods
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Dendritic Cells / immunology*
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Dendritic Cells / metabolism*
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Histocompatibility Antigens Class I / metabolism
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Humans
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Immunophenotyping
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Lymphocyte Activation
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Minor Histocompatibility Antigens / metabolism
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Monocytes / cytology
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Monocytes / immunology
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Monocytes / metabolism
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Mucosal-Associated Invariant T Cells / immunology*
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Mucosal-Associated Invariant T Cells / metabolism*
Substances
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Antigens, Bacterial
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Biomarkers
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Histocompatibility Antigens Class I
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MR1 protein, human
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Minor Histocompatibility Antigens