Interactions Between MAIT Cells and Dendritic Cells

Methods Mol Biol. 2020:2098:125-139. doi: 10.1007/978-1-0716-0207-2_8.

Abstract

Mucosal-associated invariant T (MAIT) cells recognize intermediates of the vitamin B2 biosynthetic pathway present in a variety of bacteria, presented by the monomorphic MR1 molecules. Because of their central role in shaping adaptive immunity through interaction with dendritic cells (DCs) and B cells, their manipulation can be of translational relevance. We describe a method to routinely isolate and maintain MAIT cells from peripheral blood and to investigate their activity using DC as targets.

Keywords: CD40L; MAIT; MR1 dendritic cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antigens, Bacterial / immunology
  • Biomarkers
  • Cell Communication* / immunology
  • Cell Differentiation
  • Cell Separation / methods
  • Dendritic Cells / immunology*
  • Dendritic Cells / metabolism*
  • Histocompatibility Antigens Class I / metabolism
  • Humans
  • Immunophenotyping
  • Lymphocyte Activation
  • Minor Histocompatibility Antigens / metabolism
  • Monocytes / cytology
  • Monocytes / immunology
  • Monocytes / metabolism
  • Mucosal-Associated Invariant T Cells / immunology*
  • Mucosal-Associated Invariant T Cells / metabolism*

Substances

  • Antigens, Bacterial
  • Biomarkers
  • Histocompatibility Antigens Class I
  • MR1 protein, human
  • Minor Histocompatibility Antigens