A four-channel paper microfluidic device with gold nanoparticles and aptamers for seized drugs

Ling Wang; Bruce McCord

doi:10.1016/j.ab.2020.113619

A four-channel paper microfluidic device with gold nanoparticles and aptamers for seized drugs

Anal Biochem. 2020 Apr 15:595:113619. doi: 10.1016/j.ab.2020.113619. Epub 2020 Feb 11.

Authors

Ling Wang¹, Bruce McCord²

Affiliations

¹ Department of Chemistry and Biochemistry, Florida International University, Miami, FL, 33199, USA.
² Department of Chemistry and Biochemistry, Florida International University, Miami, FL, 33199, USA. Electronic address: mccordb@fiu.edu.

PMID: 32057728
DOI: 10.1016/j.ab.2020.113619

Abstract

In this note we describe a four-channel paper microfluidic device (μPAD) that utilizes gold nanoparticles (AuNPs) as colorimetric sensors and specifically tailored aptamers to capture a set of targeted drugs, including cocaine, codeine and methamphetamine. The design utilizes salt-induced aggregation of AuNPs to produce a color change that indicates the presence of target analytes. In the absence of the targets, the aptamers bind to AuNPs, preventing aggregation. This μPAD was developed by optimizing the concentrations of aggregating agents, AuNPs and aptamers. The resultant device is sensitive and specific, producing a positive result upon detection of the target analyte.

Keywords: Aptamers; Gold nanoparticles; Lateral flow assay; Seized drugs; μPAD.

Publication types

Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

MeSH terms

Aptamers, Nucleotide / chemistry
Cocaine / analysis*
Codeine / analysis*
Colorimetry
Gold / chemistry
Lab-On-A-Chip Devices*
Metal Nanoparticles / chemistry
Methamphetamine / analysis*
Paper*

Substances

Aptamers, Nucleotide
Methamphetamine
Gold
Cocaine
Codeine

Grants and funding

R15 DA036821/DA/NIDA NIH HHS/United States