Vitamin D metabolites influence expression of genes concerning cellular viability and function in insulin producing β-cells (INS1E)

Mette E Bornstedt; Nina Gjerlaugsen; Ole Kristoffer Olstad; Jens Petter Berg; May K Bredahl; Per M Thorsby

doi:10.1016/j.gene.2020.144649

Vitamin D metabolites influence expression of genes concerning cellular viability and function in insulin producing β-cells (INS1E)

Gene. 2020 Jul 1:746:144649. doi: 10.1016/j.gene.2020.144649. Epub 2020 Apr 3.

Authors

Mette E Bornstedt¹, Nina Gjerlaugsen², Ole Kristoffer Olstad³, Jens Petter Berg⁴, May K Bredahl², Per M Thorsby²

Affiliations

¹ Hormone Laboratory, Dep. of Medical Biochemistry, Oslo University Hospital, Norway; Institute of Clinical Medicine, Faculty of Medicine, University of Oslo, Norway. Electronic address: metbo@ous-hf.no.
² Hormone Laboratory, Dep. of Medical Biochemistry, Oslo University Hospital, Norway.
³ Dep. of Medical Biochemistry, Oslo University Hospital, Ullevål, Norway.
⁴ Institute of Clinical Medicine, Faculty of Medicine, University of Oslo, Norway; Dep. of Medical Biochemistry, Oslo University Hospital, Ullevål, Norway.

PMID: 32251702
DOI: 10.1016/j.gene.2020.144649

Abstract

Background: Studies have shown that vitamin D can enhance glucose-stimulated insulin secretion (GSIS) and change the expression of genes in pancreatic β-cells. Still the mechanisms linking vitamin D and GSIS are unknown.

Material and methods: We used an established β-cell line, INS1E. INS1E cells were pre-treated with 10 nM 1,25(OH)₂vitamin D or 10 nM 25(OH)vitamin D for 72 h and stimulated with 22 mM glucose for 60 min. RNA was extracted for gene expression analysis.

Results: Expression of genes affecting viability, apoptosis and GSIS changed after pre-treatment with both 1,25(OH)₂vitamin D and 25(OH)vitamin D in INS1E cells. Stimulation with glucose after pre-treatment of INS1E cells with 1,25(OH)₂vitamin D resulted in 181 differentially expressed genes, whereas 526 genes were differentially expressed after pre-treatment with 25(OH)vitamin D.

Conclusion: Vitamin D metabolites may affect pancreatic β-cells and GSIS through changed gene expression for genes involved in β-cell function and viability.

Keywords: Gene expression; Glucose-stimulated insulin secretion (GSIS); Vitamin D; β-Cell.

MeSH terms

Animals
Apoptosis* / drug effects
Apoptosis* / genetics
Cell Line, Tumor
Cell Survival / drug effects
Gene Expression Regulation / drug effects*
Insulin Secretion / drug effects*
Insulin-Secreting Cells / cytology
Insulin-Secreting Cells / metabolism*
Rats
Vitamin D / analogs & derivatives*
Vitamin D / pharmacokinetics
Vitamin D / pharmacology

Substances

Vitamin D
25-hydroxyvitamin D