Analysis of predicted proteasomal cleavages in the methyltransferase domain from JEV

Sarah Afaq; Arshi Malik; Md Salman Akhtar; Afaf S Alwabli; Dhafer A Alzahrani; Habeeb M Al-Solami; Othman Alzahrani; Qamre Alam; Mohammad Azhar Kamal; Aala A Abulfaraj; Mohammad Tarique

doi:10.6026/97320630016223

Analysis of predicted proteasomal cleavages in the methyltransferase domain from JEV

Bioinformation. 2020 Mar 31;16(3):223-228. doi: 10.6026/97320630016223. eCollection 2020.

Authors

Affiliations

¹ Department of Clinical Biochemistry, College of Medicine, King Khalid University, Abha, Kingdom of Saudi Arabia.
² Department of Basic Medical Sciences, Faculty of Applied Medical Sciences, Al-Baha University, Al-Baha, Kingdom of Saudi Arabia.
³ Department of Biological Sciences, Faculty of Science, King Abdulaziz University, Jeddah 21589, Kingdom of Saudi Arabia.
⁴ Department of Biology, Faculty of Science, University of Tabuk, Tabuk, Kingdom of Saudi Arabia.
⁵ Medical Genomics Research Department, King Abdullah International Medical Research Center, King Saud bin Abdulaziz University for Health Sciences, Ministry of National Guard Health Affairs, Riyadh, Saudi Arabia.
⁶ Department of Biochemistry, Faculty of Science, University of Jeddah, Jeddah, Saudi Arabia.
⁷ University of Jeddah Center for Science and Medical Research (UJC-SMR), Jeddah, Saudi Arabia.
⁸ Department of Biology, Science and Arts-Rabigh Campus, King Abdulaziz University, Jeddah, Saudi Arabia.
⁹ Center for Interdisciplinary Research in Basic Sciences, Jamia Millia Islamia, Jamia Nagar, New Delhi-110025, India.

Abstract

The methyltransferase (MTase, a 265 amino acid residues long region at the N-terminal end of the viral nonfunctional supermolecule NS5 domain) is key for viral replication in Japanese Encephalitis Virus (JEV). Sequence to structure to functional information with adequate knowledge on MTase from JEV is currently limited. Therefore, it is of interest to document a report on the comprehensive analysis of predicted proteasomal cleavage data in the methyltransferase domain from JEV. This data is relevant in the design and development of vaccine and other therapeutic candidates for further consideration.

Keywords: Japanese encephalitis (JEV); cleavage; infection; methyltransferase; proteasome.