In vitro release, stability as well as bioavailability of sulphamethoxazole (SMZ) suppositories were investigated using solid dispersion techniques for surfactant incorporation. Tween 20, Tween 80 and Myrj 53 were the surfactants utilized in this investigation. Suppositories were prepared by fusion method using Witepsol H15 as a base. It was found that SMZ-surfactant physical mixture showed the highest drug release from suppositories. This indicated that the technique of surfactant incorporation has a great role in enhancing the drug release from suppositories. Stability study revealed that the technique of surfactant incorporation did not affect the drug stability. Bioavailability investigation has proved surfactant incorporation in suppository formulations of SMZ in fatty bases.