Preservation of global synaptic excitatory to inhibitory ratio during long postmortem intervals

Sci Rep. 2020 May 25;10(1):8626. doi: 10.1038/s41598-020-65377-3.

Abstract

The study of postsynaptic excitation to inhibition (E/I ratio) imbalances in human brain diseases, is a highly relevant functional measurement poorly investigated due to postmortem degradation of synaptic receptors. We show that near-simultaneous recording of microtransplanted synaptic receptors after simulated morgue conditions allows the determination of the postsynaptic E/I ratio for at least 120 h after death, expanding the availability and use of human diseased tissue stored in brain banks.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Animals
  • Brain / physiology*
  • Brain Diseases / pathology
  • Excitatory Postsynaptic Potentials / drug effects
  • Excitatory Postsynaptic Potentials / physiology*
  • Female
  • Humans
  • Inhibitory Postsynaptic Potentials / drug effects
  • Inhibitory Postsynaptic Potentials / physiology*
  • Kainic Acid / pharmacology
  • Male
  • Oocytes / cytology
  • Oocytes / metabolism
  • Rats
  • Rats, Wistar
  • Receptors, Neurotransmitter / metabolism
  • Synaptosomes / drug effects
  • Synaptosomes / physiology
  • Temperature
  • Time Factors
  • gamma-Aminobutyric Acid / pharmacology

Substances

  • Receptors, Neurotransmitter
  • gamma-Aminobutyric Acid
  • Kainic Acid