The Ubiquitin E3 Ligase PUB17 Positively Regulates Immunity by Targeting a Negative Regulator, KH17, for Degradation

Plant Commun. 2020 Jul 13;1(4):100020. doi: 10.1016/j.xplc.2020.100020.

Abstract

Ubiquitination is a post-translational modification that regulates many processes in plants. Several ubiquitin E3 ligases act as either positive or negative regulators of immunity by promoting the degradation of different substrates. StPUB17 is an E3 ligase that has previously been shown to positively regulate immunity to bacteria, fungi and oomycetes, including the late blight pathogen Phytophthora infestans. Silencing of StPUB17 promotes pathogen colonization and attenuates Cf4/avr4 cell death. Using yeast-2-hybrid and co-immunoprecipitation we identified the putative K-homology (KH) RNA-binding protein (RBP), StKH17, as a candidate substrate for degradation by StPUB17. StKH17 acts as a negative regulator of immunity that promotes P. infestans infection and suppresses specific immune pathways. A KH RBP domain mutant of StKH17 (StKH17GDDG) is no longer able to negatively regulate immunity, indicating that RNA binding is likely required for StKH17 function. As StPUB17 is a known target of the ubiquitin E3 ligase, StPOB1, we reveal an additional step in an E3 ligase regulatory cascade that controls plant defense.

Keywords: E3 ligase; KH RNA-binding protein; late blight; oomycete; plant disease.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Death
  • Gene Expression Regulation, Plant / immunology*
  • Nicotiana / genetics*
  • Nicotiana / immunology
  • Plant Immunity / genetics*
  • Plant Proteins / genetics*
  • Plant Proteins / immunology
  • Ubiquitin-Protein Ligases / genetics*
  • Ubiquitin-Protein Ligases / immunology
  • Ubiquitination

Substances

  • Plant Proteins
  • Ubiquitin-Protein Ligases