LPS-Inducible lncRNA TMC3-AS1 Negatively Regulates the Expression of IL-10

Front Immunol. 2020 Jul 22:11:1418. doi: 10.3389/fimmu.2020.01418. eCollection 2020.

Abstract

Long non-coding RNAs are essential regulators of the inflammatory response, especially for transcriptional regulation of inflammatory genes. It has been reported that the expression of transmembrane channel-like 3 (TMC3)-AS1 is increased following lipopolysaccharide stimulation. However, the potential function of TMC3-AS1 in immunity is largely unknown. Herein, we report a specific role for TMC3-AS1 in the regulation of inflammatory gene expression. TMC3-AS1 negatively regulates the expression of interleukin 10 (IL-10) in macrophage and intestinal epithelial cell lines. Mechanistically, TMC3-AS1 may interact with p65 in the nucleus, preventing p65 from binding to the κB consensus site within IL-10 promoter. These findings suggest that TMC3-AS1 may function as an important regulator in the innate immune response.

Keywords: IL-10; NF-κB; TLR4; TMC3-AS1; innate immunity.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Gene Expression Regulation / genetics*
  • Humans
  • Immunity, Innate / immunology
  • Inflammation / genetics
  • Inflammation / immunology
  • Interleukin-10 / biosynthesis*
  • Intestinal Mucosa / immunology*
  • Intestinal Mucosa / metabolism
  • Ion Channels
  • Lipopolysaccharides / pharmacology
  • Macrophages / immunology*
  • Macrophages / metabolism
  • Mice
  • RNA, Long Noncoding / metabolism*

Substances

  • Ion Channels
  • Lipopolysaccharides
  • RNA, Long Noncoding
  • Interleukin-10