Protocol for the Standardized Generation of Forward Programmed Cryopreservable Excitatory and Inhibitory Forebrain Neurons

Michael Peitz; Tamara Krutenko; Oliver Brüstle

doi:10.1016/j.xpro.2020.100038

Protocol for the Standardized Generation of Forward Programmed Cryopreservable Excitatory and Inhibitory Forebrain Neurons

STAR Protoc. 2020 Jun 3;1(1):100038. doi: 10.1016/j.xpro.2020.100038. eCollection 2020 Jun 19.

Authors

Michael Peitz^{1

2}, Tamara Krutenko¹, Oliver Brüstle¹

Affiliations

¹ Institute of Reconstructive Neurobiology, University of Bonn Medical Faculty & University Hospital Bonn, Bonn, Germany.
² Cell Programming Core Facility, University of Bonn Medical Faculty, Bonn, Germany.

Abstract

This protocol describes a highly standardized pipeline for transcription factor-mediated forward programming of human pluripotent stem cells into highly enriched glutamatergic or GABAergic neurons followed by a cryopreservation step that enables the generation of large quality-controlled batches. This approach is particularly useful for reducing interexperimental variability in the context of collaborative studies across different locations and time points. For complete details on the use and execution of this protocol, please refer to Meijer et al. (2019) and Rhee et al. (2019).

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cell Culture Techniques / methods*
Cell Differentiation
Cells, Cultured
Cryopreservation / methods*
Humans
Neurons / cytology*
Pluripotent Stem Cells / cytology*
Prosencephalon / cytology*