Assessment of Osteocytes: Techniques for Studying Morphological and Molecular Changes Associated with Perilacunar/Canalicular Remodeling of the Bone Matrix

Neha S Dole; Cristal S Yee; Charles A Schurman; Sarah L Dallas; Tamara Alliston

doi:10.1007/978-1-0716-1028-2_17

Assessment of Osteocytes: Techniques for Studying Morphological and Molecular Changes Associated with Perilacunar/Canalicular Remodeling of the Bone Matrix

Methods Mol Biol. 2021:2230:303-323. doi: 10.1007/978-1-0716-1028-2_17.

Authors

Neha S Dole¹, Cristal S Yee¹, Charles A Schurman^{1

2}, Sarah L Dallas³, Tamara Alliston^{4

5}

Affiliations

¹ Department of Orthopaedic Surgery, University of California, San Francisco, San Francisco, CA, USA.
² UC Berkeley/UCSF Graduate Program in Bioengineering, San Francisco, CA, USA.
³ Department of Oral and Craniofacial Sciences, School of Dentistry, University of Missouri Kansas City, Kansas City, MO, USA.
⁴ Department of Orthopaedic Surgery, University of California, San Francisco, San Francisco, CA, USA. tamara.alliston@ucsf.edu.
⁵ UC Berkeley/UCSF Graduate Program in Bioengineering, San Francisco, CA, USA. tamara.alliston@ucsf.edu.

Abstract

Recent advances have revived interest in the concept of osteocyte perilacunar/canalicular remodeling (PLR) and have motivated efforts to identify the mechanisms regulating this process in bone in the context of normal physiology and pathological conditions. Here, we describe several methods that are evaluating morphological changes associated with PLR function of osteocytes.

Keywords: Lacunocanalicular network; Osteocyte techniques; Osteocytes; Perilacunar/canalicular remodeling.

Publication types

Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Animals
Bone Matrix / ultrastructure*
Bone Remodeling / physiology*
Cell Culture Techniques / methods*
Humans
Osteocytes / ultrastructure*

Abstract

Publication types

MeSH terms

Grants and funding