Complementary and synergistic effects on osteogenic and angiogenic properties of copper-incorporated silicocarnotite bioceramic: In vitro and in vivo studies

Qiang Wu; Shunxiang Xu; Xin Wang; Bo Jia; Yu Han; Yifu Zhuang; Ye Sun; Zhenyu Sun; Yaping Guo; Huamin Kou; Congqin Ning; Kerong Dai

doi:10.1016/j.biomaterials.2020.120553

Complementary and synergistic effects on osteogenic and angiogenic properties of copper-incorporated silicocarnotite bioceramic: In vitro and in vivo studies

Biomaterials. 2021 Jan:268:120553. doi: 10.1016/j.biomaterials.2020.120553. Epub 2020 Nov 24.

Authors

Qiang Wu¹, Shunxiang Xu², Xin Wang³, Bo Jia¹, Yu Han¹, Yifu Zhuang¹, Ye Sun¹, Zhenyu Sun¹, Yaping Guo⁴, Huamin Kou⁵, Congqin Ning⁶, Kerong Dai⁷

Affiliations

¹ Shanghai Key Laboratory of Orthopaedic Implant, Department of Orthopaedic Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
² The Education Ministry Key Lab of Resource Chemistry and Shanghai Key Laboratory of Rare Earth Functional Materials, Shanghai Normal University, Shanghai, China; State Key Laboratory of High Performance Ceramics and Superfine Microstructure, Shanghai Institute of Ceramics, Chinese Academy of Sciences, Shanghai, China.
³ Department of Orthopedics Trauma and Microsurgery, Zhongnan Hospital of Wuhan University, Wuhan, China.
⁴ The Education Ministry Key Lab of Resource Chemistry and Shanghai Key Laboratory of Rare Earth Functional Materials, Shanghai Normal University, Shanghai, China.
⁵ State Key Laboratory of High Performance Ceramics and Superfine Microstructure, Shanghai Institute of Ceramics, Chinese Academy of Sciences, Shanghai, China.
⁶ The Education Ministry Key Lab of Resource Chemistry and Shanghai Key Laboratory of Rare Earth Functional Materials, Shanghai Normal University, Shanghai, China; State Key Laboratory of High Performance Ceramics and Superfine Microstructure, Shanghai Institute of Ceramics, Chinese Academy of Sciences, Shanghai, China. Electronic address: cqning@shnu.edu.cn.
⁷ Shanghai Key Laboratory of Orthopaedic Implant, Department of Orthopaedic Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China. Electronic address: krdai@163.com.

PMID: 33253963
DOI: 10.1016/j.biomaterials.2020.120553

Abstract

Promoting bone regeneration to treat bone defects is a challenging problem in orthopedics, and developing novel biomaterials with both osteogenic and angiogenic activities is sought as a feasible solution. Here, copper-silicocarnotite [Cu-Ca₅(PO₄)₂SiO₄, Cu-CPS] was designed and fabricated. In this study, the Cu-CPS ceramics demonstrated better mechanical, osteogenic, and angiogenic properties in vitro and in vivo than pure CPS one. Particularly, CPS with 1.0 wt% CuO (1.0Cu-CPS) exhibited the best performance. Additionally, hydroxyapatite with 1.0 wt% CuO (1.0Cu-HA) was used to explore the respective effects of copper and silicon (Si). According to the in vitro results, it indicated that Cu enhanced the osteogenic activity of CPS ceramics although Si played a dominate role in the osteogenic process. Moreover, Cu could promote an early stage of angiogenesis, and the complementary effect of Si and Cu was found in the late phase. Furthermore, the in vivo results illustrated that the synergistic effect of Cu and Si improved bone and vessel regeneration during the degradation of Cu-CPS scaffolds (P < 0.05). Therefore, Cu-CPS ceramics could improve osteogenesis and angiogenesis through the simultaneous effects of Cu and Si, thus, offering a promising treatment option in orthopedic application for bone tissue regeneration.

Keywords: Angiogenesis; Copper; Mechanical property; Osteogenesis; Silicate ceramics; Synergistic effect.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Bone Regeneration
Calcium Phosphates
Ceramics / pharmacology
Copper*
Osteogenesis*
Silicates / pharmacology

Substances

Calcium Phosphates
Silicates
silicocarnotite
Copper