Characterization of T-cell receptor transgenic mice recognizing immunodominant HLA-DQ2.5-restricted gluten epitopes

Christian B Lindstad; Shuo-Wang Qiao; Marie K Johannesen; Lars Fugger; Ludvig M Sollid; M Fleur du Pré

doi:10.1002/eji.202048859

Characterization of T-cell receptor transgenic mice recognizing immunodominant HLA-DQ2.5-restricted gluten epitopes

Eur J Immunol. 2021 Apr;51(4):1002-1005. doi: 10.1002/eji.202048859. Epub 2020 Dec 28.

Authors

Christian B Lindstad^{1

2}, Shuo-Wang Qiao^{1

2}, Marie K Johannesen^{1

2}, Lars Fugger³, Ludvig M Sollid^{1

2

4}, M Fleur du Pré^{1

4}

Affiliations

¹ K.G. Jebsen Coeliac Disease Research Centre, University of Oslo, Oslo, Norway.
² Department of Immunology, University of Oslo, Oslo, Norway.
³ Oxford Centre for Neuroinflammation, Nuffield Department of Clinical Neurosciences, Division of Clinical Neurology and Medical Research Council Human Immunology Unit, Weatherall Institute of Molecular Medicine, John Radcliffe Hospital, University of Oxford, Oxford, UK.
⁴ Department of Immunology, Oslo University Hospital, Oslo, Norway.

PMID: 33368209
DOI: 10.1002/eji.202048859

Abstract

We created a TCR transgenic mouse with CD4⁺ T cells recognizing the immunodominant DQ2.5-glia-ω2 gluten epitope. We show that these cells respond to deamidated gluten feed in vivo and compare them to previously published α2- and γ1-specific mice. These mice may help enlighten key aspects of celiac disease pathogenesis.

Keywords: HLA-DQ2.5; TCR; celiac; gluten; mouse.

Publication types

Letter
Research Support, Non-U.S. Gov't

MeSH terms

Animals
CD4-Positive T-Lymphocytes / immunology
CD4-Positive T-Lymphocytes / metabolism
Celiac Disease / genetics
Celiac Disease / immunology
Disease Models, Animal
Glutens / genetics*
Glutens / immunology
HLA-DQ Antigens / genetics*
HLA-DQ Antigens / immunology
Humans
Immunodominant Epitopes / genetics*
Immunodominant Epitopes / immunology
Mice
Mice, Transgenic
Receptors, Antigen, T-Cell / genetics*
Receptors, Antigen, T-Cell / immunology

Substances

HLA-DQ Antigens
HLA-DQ2 antigen
Immunodominant Epitopes
Receptors, Antigen, T-Cell
Glutens