Antinociceptive and anti-inflammatory activity of α-d-mannan from Pseudozyma sp

Gildomar Lima Valasques Junior; Jener David Gonçalves Dos Santos; Pedro Felipe Pereira Chaves; Lucimara Mach Côrtes Cordeiro; Cleisiane Lima de Jesus; Flávia Oliveira de Lima; Elisangela Fabiana Boffo; Sandra Aparecida de Assis

doi:10.1007/s13205-020-02635-1

Antinociceptive and anti-inflammatory activity of α-d-mannan from Pseudozyma sp

3 Biotech. 2021 Feb;11(2):73. doi: 10.1007/s13205-020-02635-1. Epub 2021 Jan 13.

Authors

Gildomar Lima Valasques Junior¹, Jener David Gonçalves Dos Santos², Pedro Felipe Pereira Chaves³, Lucimara Mach Côrtes Cordeiro³, Cleisiane Lima de Jesus⁴, Flávia Oliveira de Lima⁴, Elisangela Fabiana Boffo⁵, Sandra Aparecida de Assis¹

Affiliations

¹ Enzymology and Fermentation Technology Laboratory, Health Department, State University of Feira de Santana, Av Transnordestina, km 0, BR 116, Feira de Santana, BA CEP 44036-900 Brazil.
² University Veterinary Hospital, Federal University of Agreste of Pernambuco, Garanhuns, PE Brazil.
³ Biochemistry and Molecular Biology Department, Federal University of Paraná, CP 19.046, Curitiba, PR CEP 81531-980 Brazil.
⁴ Health Department, State University of Feira de Santana, Feira de Santana, CEP 44036-900 Bahia Brazil.
⁵ Department of Organic Chemistry, Institute of Chemistry, Universidade Federal da Bahia, Campus Universitário de Ondina, Salvador, BA 40170-115 Brazil.

Abstract

Pseudozyma sp. are yeasts that are commercially important due to their production of glycolipid biosurfactants, squalene, itaconic acid, and exopolysaccharide. The search for other analgesia inducing drugs, such as opiates and non-steroidal anti-inflammatory drugs (NSAIDs), as alternatives is beneficial. In this study, the antinociceptive and anti-inflammatory actions of α-d-mannan were studied using acetic acid-induced writhing, open field test, formalin test, and carrageenan-induced paw oedema tests in mice. The α-d-mannan obtained from Pseudozyma sp. was confirmed by methylation analysis, 1D and 2D NMR spectroscopic analysis, and GC-MS. The results show that α-d-mannan from Pseudozyma sp. has analgesic and anti-inflammatory activities.

Supplementary information: The online version contains supplementary material available at 10.1007/s13205-020-02635-1.

Keywords: Anti-inflammatory; Antinociceptive; Pseudozyma sp.; Yeast; α-d-mannan.