Protocol for mouse adult neural stem cell isolation and culture

Ahmed K M A Ahmed; Toke Jost Isaksen; Toshihide Yamashita

doi:10.1016/j.xpro.2021.100522

Protocol for mouse adult neural stem cell isolation and culture

STAR Protoc. 2021 May 4;2(2):100522. doi: 10.1016/j.xpro.2021.100522. eCollection 2021 Jun 18.

Authors

Ahmed K M A Ahmed^{1

2}, Toke Jost Isaksen¹, Toshihide Yamashita^{1

2

3

4}

Affiliations

¹ Department of Molecular Neuroscience, Graduate School of Medicine, Osaka University, 2-2, Yamadaoka, Suita, Osaka 565-0871, Japan.
² WPI Immunology Frontier Research Center, Osaka University, 3-1, Yamadaoka, Suita, Osaka 565-0871, Japan.
³ Graduate School of Frontier Bioscience, Osaka University, 2-2, Yamadaoka, Suita, Osaka 565-0871, Japan.
⁴ Department of Neuro-Medical Science, Graduate School of Medicine, Osaka University, 2-2, Yamadaoka, Suita, Osaka 565-0871, Japan.

Abstract

This protocol entails a simple method for isolation, culturing, and in vitro differentiation of adult neural stem cells from the dentate gyrus in the hippocampus and the subventricular zone of adult mice. Cultured adult neural stem cells are an important in vitro model to investigate stem cell properties such as proliferation and differentiation and to expand the understanding of plasticity in the adult brain. For complete details on the use and execution of this protocol, please refer to Isaksen et al. (2020).

Keywords: Cell Differentiation; Cell culture; Cell isolation; Neuroscience; Stem Cells.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Brain / cytology
Cell Culture Techniques / methods*
Cell Separation / methods*
Cells, Cultured
Dissection
Mice
Neural Stem Cells / cytology*