Generation of induced pluripotent stem cell line (ZZUi027-A) derived from skin fibroblasts from a Parkinson's disease patient with RAB39B gene mutation

Huifang Sun; Zhuoya Wang; Qi Zhang; Yutao Liu; Jing Yang; Han Liu; Changhe Shi; Yanlin Wang; Yuming Xu

doi:10.1016/j.scr.2021.102454

Generation of induced pluripotent stem cell line (ZZUi027-A) derived from skin fibroblasts from a Parkinson's disease patient with RAB39B gene mutation

Stem Cell Res. 2021 Aug:55:102454. doi: 10.1016/j.scr.2021.102454. Epub 2021 Jul 4.

Authors

Huifang Sun¹, Zhuoya Wang², Qi Zhang², Yutao Liu², Jing Yang², Han Liu², Changhe Shi², Yanlin Wang³, Yuming Xu⁴

Affiliations

¹ Department of Neurology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou University, Zhengzhou, Henan 450052, China; School of Medical Sciences, Zhengzhou University, Zhengzhou, Henan 450001, China.
² Department of Neurology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou University, Zhengzhou, Henan 450052, China.
³ Department of Neurology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou University, Zhengzhou, Henan 450052, China. Electronic address: wyllyh1986@126.com.
⁴ Department of Neurology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou University, Zhengzhou, Henan 450052, China. Electronic address: xuyuming@zzu.edu.cn.

PMID: 34247111
DOI: 10.1016/j.scr.2021.102454

Abstract

Parkinson's disease is a common neurogenetic degenerative disease that can be caused by a variety of genetic mutations. RAB39B gene mutations have recently been identified as a cause of Parkinson's disease. We collected skin tissue samples from a family with mutations in RAB39B for our clinical study. Additionally, we constructed patient-derived induced pluripotent stem cells (iPSCs) from the patient's uncle using an unintegrated reprogramming plasmid transfection method to create a reliable cell model for the subsequent study of Parkinson's disease.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Fibroblasts
Humans
Induced Pluripotent Stem Cells*
Mutation
Parkinson Disease* / genetics
rab GTP-Binding Proteins / genetics

Substances

Rab39B protein, human
rab GTP-Binding Proteins