Transcriptomic Analysis of Gene Networks Regulated by U11 Small Nuclear RNA in Bladder Cancer

Zhenxing Wang; Xi Wang; Yaobang Wang; Shaomei Tang; Chao Feng; Lixin Pan; Qinchen Lu; Yuting Tao; Yuanliang Xie; Qiuyan Wang; Zhong Tang

doi:10.3389/fgene.2021.695597

Transcriptomic Analysis of Gene Networks Regulated by U11 Small Nuclear RNA in Bladder Cancer

Front Genet. 2021 Jul 2:12:695597. doi: 10.3389/fgene.2021.695597. eCollection 2021.

Authors

Zhenxing Wang^{1

2

3}, Xi Wang^{1

2}, Yaobang Wang^{1

2}, Shaomei Tang⁴, Chao Feng^{1

2}, Lixin Pan^{1

2}, Qinchen Lu^{1

2}, Yuting Tao^{1

2}, Yuanliang Xie^{1

2

5}, Qiuyan Wang^{1

2}, Zhong Tang^{1

2

6}

Affiliations

¹ Center for Genomic and Personalized Medicine, Guangxi Medical University, Nanning, China.
² Guangxi Key Laboratory for Genomic and Personalized Medicine, Guangxi Collaborative Innovation Center for Genomic and Personalized Medicine, Nanning, China.
³ Department of Clinical Laboratory, The First Affiliated Hospital of Guangxi Medical University, Nanning, China.
⁴ Department of Gastroenterology, The First Affiliated Hospital of Guangxi Medical University, Nanning, China.
⁵ Department of Urology, The Affiliated Cancer Hospital of Guangxi Medical University, Nanning, China.
⁶ School of Information and Management, Guangxi Medical University, Nanning, China.

Abstract

Small nuclear RNA is a class of non-coding RNA that widely exist in the nucleus of eukaryotes. Accumulated evidences have shown that small nuclear RNAs are associated with the regulation of gene expression in various tumor types. To explore the gene expression changes and its potential effects mediated by U11 snRNA in bladder cancer cells, U11 snRNA knockout and overexpressed cell lines were constructed and further used to analyze the gene expression changes by RNA sequencing. The differentially expressed genes were found to be mainly enriched in tumor-related pathways both in the U11 knockout and overexpression cell lines, such as NF-kappa B signaling pathway, bladder cancer and PI3K-Akt signaling pathway. Furthermore, alternative splicing events were proposed to participate in the potential regulatory mechanism induced by the U11 knockout or overexpression. In conclusion, U11 may be involved in the regulation of gene expression in bladder cancer cells, which may provide a potentially new biomarker for clinical diagnosis and treatment of bladder cancer.

Keywords: T24; U11 small nuclear RNA; bladder cancer; gene networks; transcriptomic analysis.