The current study investigated the antioxidant capacity of enzymatically cleaved low acyl gellan gum (LA-GAGR) fragments, named midi-GAGR (MWv : 1.2 × 105 Da) and mini-GAGR (MWv : 2.5 × 104 Da). Three different methods-hydroxide assay, superoxide assay, and DPPH assay-were used to measure the antioxidant capacity of the low acyl gellan gum fragments. Both mini-GAGR and midi-GAGR showed similar antioxidant capacities, 27.1% and 25.6%, respectively, for hydroxide radicals, whereas ascorbic acid showed 9.8%. For superoxide radicals, the fragments scavenged 41.7% (mini) and 35.6% (midi) of free radicals compared to 10.6% removal by ascorbic acid. Mini- and midi-GAGR displayed modest scavenging capabilities with DPPH radicals (8.5% and 6.6%, respectively) as compared to ascorbic acid (96.3%). Both midi- and mini-GAGR showed less gel-like behaviors than LA-GAGR. Midi-GAGR was observed to have a transition from liquid to gel at 63 rad/s. PRACTICAL APPLICATION: The results in the manuscript are helpful when gellan gum and its derivatives are directly applied to food processing as a dietary fiber supplement or a stabilizer for functional beverages. The antioxidant capacity results can be used to promote the functionality of gellan gum as a food additive and for controlling cell adhesion and growth on gellan gum scaffolds. The rheology results will be useful for synthesis of scaffolds for bone tissue generation and facilitating clinical treatments when gellan gum is injected as an adsorbent or a filler for treating bone fractures. In the pharmaceutical industry, they are useful when controlling the therapeutic effects of drug delivery systems.
Keywords: antioxidant capacity; enzymatic hydrolysis; low acyl gellan gum; polysaccharide; rheology.
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