Effect of Enterotoxigenic Escherichia coli on Microbial Communities during Kimchi Fermentation

J Microbiol Biotechnol. 2021 Nov 28;31(11):1552-1558. doi: 10.4014/jmb.2108.08038.

Abstract

The diverse microbial communities in kimchi are dependent on fermentation period and temperature. Here, we investigated the effect of enterotoxigenic Escherichia coli (ETEC) during the fermentation of kimchi at two temperatures using high-throughput sequencing. There were no differences in pH between the control group, samples not inoculated with ETEC, and the ETEC group, samples inoculated with ETEC MFDS 1009477. The pH of the two groups, which were fermented at 10 and 25°C, decreased rapidly at the beginning of fermentation and then reached pH 3.96 and pH 3.62. In both groups, the genera Lactobacillus, Leuconostoc, and Weissella were predominant. Our result suggests that microbial communities during kimchi fermentation may be affected by the fermentation parameters, such as temperature and period, and not enterotoxigenic E. coli (ETEC).

Keywords: 16S rRNA gene; Enterotoxigenic E. coli (ETEC); Kimchi; Microbial community.

MeSH terms

  • Brassica
  • Enterotoxigenic Escherichia coli*
  • Fermentation
  • Fermented Foods / microbiology*
  • Food Microbiology*
  • High-Throughput Nucleotide Sequencing
  • Hydrogen-Ion Concentration
  • Lactobacillus / classification
  • Leuconostoc / classification
  • Microbiota*
  • RNA, Ribosomal, 16S / genetics
  • Temperature
  • Weissella / classification

Substances

  • RNA, Ribosomal, 16S