Partial Opening of Cytochrome P450cam (CYP101A1) Is Driven by Allostery and Putidaredoxin Binding

Biochemistry. 2021 Oct 5;60(39):2932-2942. doi: 10.1021/acs.biochem.1c00406. Epub 2021 Sep 14.

Abstract

Cytochrome P450cam (CYP101A1) catalyzes the regio- and stereo-specific 5-exo-hydroxylation of camphor via a multistep catalytic cycle that involves two-electron transfer steps, with an absolute requirement that the second electron be donated by the ferrodoxin, putidaredoxin (Pdx). Whether P450cam, once camphor has bound to the active site and the substrate entry channel has closed, opens up upon Pdx binding, during the second electron transfer step, or it remains closed is still a matter of debate. A potential allosteric site for camphor binding has been identified and postulated to play a role in the binding of Pdx. Here, we have revisited paramagnetic NMR spectroscopy data and determined a heterogeneous ensemble of structures that explains the data, provides a complete representation of the P450cam/Pdx complex in solution, and reconciles alternative hypotheses. The allosteric camphor binding site is always present, and the conformational changes induced by camphor binding to this site facilitates Pdx binding. We also determined that the state to which Pdx binds comprises an ensemble of structures that have features of both the open and closed state. These results demonstrate that there is a finely balanced interaction between allosteric camphor binding and the binding of Pdx at high camphor concentrations.

MeSH terms

  • Allosteric Regulation
  • Bacterial Proteins / chemistry*
  • Bacterial Proteins / metabolism*
  • Camphor / chemistry*
  • Camphor / metabolism
  • Camphor 5-Monooxygenase / chemistry*
  • Camphor 5-Monooxygenase / metabolism*
  • Catalytic Domain
  • Crystallography, X-Ray / methods
  • Ferredoxins / metabolism*
  • Magnetic Resonance Spectroscopy / methods
  • Models, Molecular
  • Protein Binding
  • Protein Conformation
  • Pseudomonas putida / chemistry
  • Pseudomonas putida / enzymology*

Substances

  • Bacterial Proteins
  • Ferredoxins
  • putidaredoxin
  • Camphor
  • Camphor 5-Monooxygenase