Nitrosomonas supralitoralis sp. nov., an ammonia-oxidizing bacterium from beach sand in a supralittoral zone

Hidetoshi Urakawa; Gabrianna A Andrews; Jose V Lopez; Willm Martens-Habbena; Martin G Klotz; David A Stahl

doi:10.1007/s00203-022-03173-5

Nitrosomonas supralitoralis sp. nov., an ammonia-oxidizing bacterium from beach sand in a supralittoral zone

Arch Microbiol. 2022 Aug 17;204(9):560. doi: 10.1007/s00203-022-03173-5.

Authors

Hidetoshi Urakawa^{1

2}, Gabrianna A Andrews³, Jose V Lopez⁴, Willm Martens-Habbena^{5

6}, Martin G Klotz⁷, David A Stahl⁶

Affiliations

¹ Department of Ecology and Environmental Studies, Florida Gulf Coast University, Fort Myers, FL, USA. hurakawa@fgcu.edu.
² Department of Civil and Environmental Engineering, University of Washington, Seattle, WA, USA. hurakawa@fgcu.edu.
³ Department of Ecology and Environmental Studies, Florida Gulf Coast University, Fort Myers, FL, USA.
⁴ Department of Biological Sciences, Halmos College of Arts and Sciences, Nova Southeastern University, Dania Beach, FL, USA.
⁵ Fort Lauderdale Research and Education Center, Institute for Food and Agricultural Sciences, University of Florida, Davie, FL, USA.
⁶ Department of Civil and Environmental Engineering, University of Washington, Seattle, WA, USA.
⁷ School of Molecular Biosciences, Washington State University, Pullman, WA, USA.

PMID: 35978059
DOI: 10.1007/s00203-022-03173-5

Abstract

A betaproteobacterial chemolithotrophic ammonia-oxidizing bacterium designated APG5^T was isolated from supralittoral sand of the Edmonds City Beach, WA, USA. Growth was observed at 10-35 °C (optimum, 30 °C), pH 5-9 (optimum, pH 8) and ammonia concentrations as high as 100 mM (optimum, 1-30 mM NH₄Cl). The strain grows optimally in a freshwater medium but tolerates up to 400 mM NaCl. It is most closely related to 'Nitrosomonas ureae' (96.7% 16S rRNA and 92.4% amoA sequence identity). The 3.75-Mbp of AGP5^T draft genome contained a single rRNA operon and all necessary tRNA genes and has the lowest G+C content (43.5%) when compared to the previously reported genomes of reference strains in cluster 6 Nitrosomonas. Based on an average nucleotide identity of 82% with its closest relative ('N. ureae' Nm10^T) and the suggested species boundary of 95-96%, a new species Nitrosomonas supralitoralis sp. nov. is proposed. The type strain of Nitrosomonas supralitoralis is APG5^T (= NCIMB 14870^T = ATCC TSD-116^T).

Keywords: AOB; Ammonia-oxidizing bacteria; Beach; Brackish; Nitrosomonas; Sand.

MeSH terms

Ammonia*
DNA, Bacterial / chemistry
DNA, Bacterial / genetics
Nitrosomonas / genetics
Oxidation-Reduction
Phylogeny
RNA, Ribosomal, 16S / genetics
Sand*
Sequence Analysis, DNA

Substances

DNA, Bacterial
RNA, Ribosomal, 16S
Sand
Ammonia

Abstract

MeSH terms

Substances

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