Pharmacogenomic Screening of Drug Candidates using Patient-Specific hiPSC-Derived Cardiomyocyte High-Throughput Calcium Imaging

Methods Mol Biol. 2022:2547:241-253. doi: 10.1007/978-1-0716-2573-6_10.

Abstract

Calcium imaging is an invaluable technique to detect and characterize calcium flux in cells. The use of calcium dye provides information on the concentration and spatial distribution of calcium. Calcium imaging is a well-established technique to assess the calcium-induced calcium release mechanism in cardiomyocytes. It can also be used to characterize mutations in genes crucial for this mechanism that frequently causes arrhythmia. Here we describe a high-throughput methodology of calcium imaging that records individual calcium transients in more than 10,000 human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) in less than 30 min.

Keywords: Arrhythmia; Calcium imaging; Drug screening; High-throughput; hiPSC-derived cardiomyocytes.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, N.I.H., Intramural

MeSH terms

  • Arrhythmias, Cardiac
  • Calcium
  • Cell Differentiation
  • Humans
  • Induced Pluripotent Stem Cells*
  • Myocytes, Cardiac
  • Pharmacogenomic Testing

Substances

  • Calcium