Live Cell Imaging of Yeast Golgi Dynamics

Giancarlo Costaguta; Gregory S Payne; Lydia Daboussi

doi:10.1007/978-1-0716-2639-9_1

Live Cell Imaging of Yeast Golgi Dynamics

Methods Mol Biol. 2023:2557:3-15. doi: 10.1007/978-1-0716-2639-9_1.

Authors

Giancarlo Costaguta¹, Gregory S Payne², Lydia Daboussi³

Affiliations

¹ Department of Neurobiology, David Geffen School of Medicine, University of California, Los Angeles, CA, USA.
² Department of Biological Chemistry, David Geffen School of Medicine, University of California, Los Angeles, CA, USA.
³ Department of Neurobiology, David Geffen School of Medicine, University of California, Los Angeles, CA, USA. ldaboussi@mednet.ucla.edu.

PMID: 36512205
DOI: 10.1007/978-1-0716-2639-9_1

Abstract

Fluorescence imaging of live cells allows for the observation of dynamic processes inside cells in real time. Here we describe a strategy to image clathrin-coated vesicle dynamics in a single focal plane at the trans-Golgi network of the yeast Saccharomyces cerevisiae. This method can be readily adapted for live cell imaging of a diverse set of dynamic processes within cells.

Keywords: Adaptor; CCV; Clathrin; Dynamic; Golgi; Intracellular; Live cell fluorescence; Spinning disc confocal microscopy; TGN; Vesicle; Yeast.

Publication types

Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

MeSH terms

Clathrin
Clathrin-Coated Vesicles
Golgi Apparatus
Saccharomyces cerevisiae*
trans-Golgi Network*

Substances

Clathrin

Abstract

Publication types

MeSH terms

Substances

Grants and funding