Mass Spectrometry Approaches for Detection and Determination of Prostaglandins from Biological Samples

Methods Mol Biol. 2023:2625:299-311. doi: 10.1007/978-1-0716-2966-6_25.

Abstract

Accurate determination of prostaglandins (PGs) from biological samples is critical for understanding their biological functions and interactions during physiological and pathological processes. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) is a highly sensitive, accurate, and high-throughput approach for simultaneous detection of ultra-trace PGs from a single biological sample. Here we describe LC-MS/MS techniques and related sample pretreatment methods including both off-line and on-line SPE for the determination of PGs in biological samples.

Keywords: LC–MS/MS; Prostaglandins; Restricted access media (RAM)-based on-line SPE-LC-MS/MS; Sample preparation; Solid phase extraction.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Chromatography, High Pressure Liquid / methods
  • Chromatography, Liquid / methods
  • Prostaglandins*
  • Solid Phase Extraction / methods
  • Tandem Mass Spectrometry* / methods

Substances

  • Prostaglandins