Accurate determination of prostaglandins (PGs) from biological samples is critical for understanding their biological functions and interactions during physiological and pathological processes. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) is a highly sensitive, accurate, and high-throughput approach for simultaneous detection of ultra-trace PGs from a single biological sample. Here we describe LC-MS/MS techniques and related sample pretreatment methods including both off-line and on-line SPE for the determination of PGs in biological samples.
Keywords: LC–MS/MS; Prostaglandins; Restricted access media (RAM)-based on-line SPE-LC-MS/MS; Sample preparation; Solid phase extraction.
© 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.