The distribution of GABA-like immunoreactivity in the brain of the honeybee was investigated with antisera generated against GABA protein conjugates. The binding of the antisera in paraffin serial sections was studied with the peroxidase-antiperoxidase method. GABA-like immunoreactive fibers appeared in all main neuropile areas. The staining of the optic lobes showed pronounced stratification. The receptor cells of compound eyes, ocelli, and antennae were not labelled. Several prominent fiber tracts showed GABA-like immunoreactivity, whereas other tracts were devoid of staining. There are no major immunoreactive commissures linking the two brain hemispheres with the exception of small commissures that bridge short distances between the beta-lobes and the antennal lobes. Several fibers in the cervical connective were also labelled; some of those may descend from the suboesophageal ganglion to the thoracic ganglia. The dense reactivity seen in the optic and antennal neuropiles implies that GABA is more important in mediating local rather than more distant neural interactions.