Cytotoxic steroids from the stems of Strophanthus divaricatus

Hong-Ling Ran; Sheng-Zhuo Huang; Hao Wang; Li Yang; Cui-Juan Gai; Rui-Jun Duan; Hao-Fu Dai; Ya-Li Guan; Wen-Li Mei

doi:10.1016/j.phytochem.2023.113668

Cytotoxic steroids from the stems of Strophanthus divaricatus

Phytochemistry. 2023 Jun:210:113668. doi: 10.1016/j.phytochem.2023.113668. Epub 2023 Apr 4.

Authors

Hong-Ling Ran¹, Sheng-Zhuo Huang², Hao Wang², Li Yang², Cui-Juan Gai², Rui-Jun Duan², Hao-Fu Dai², Ya-Li Guan³, Wen-Li Mei⁴

Affiliations

¹ Key Laboratory of Research and Development of Natural Product from Li Folk Medicine of Hainan Province, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agriculture Sciences & Key Laboratory for Conservation and Utilization of Tropical Agro-bioresources of Hainan Province, Hainan Institute for Tropical Agricultural Resources, Haikou, 571101, PR China; Ministry of Education Key Laboratory for Ecology of Tropical Islands, Key Laboratory of Tropical Animal and Plant Ecology of Hainan Province, College of Life Sciences, Hainan Normal University, Haikou, 571158, PR China.
² Key Laboratory of Research and Development of Natural Product from Li Folk Medicine of Hainan Province, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agriculture Sciences & Key Laboratory for Conservation and Utilization of Tropical Agro-bioresources of Hainan Province, Hainan Institute for Tropical Agricultural Resources, Haikou, 571101, PR China.
³ Ministry of Education Key Laboratory for Ecology of Tropical Islands, Key Laboratory of Tropical Animal and Plant Ecology of Hainan Province, College of Life Sciences, Hainan Normal University, Haikou, 571158, PR China. Electronic address: 544758563@qq.com.
⁴ Key Laboratory of Research and Development of Natural Product from Li Folk Medicine of Hainan Province, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agriculture Sciences & Key Laboratory for Conservation and Utilization of Tropical Agro-bioresources of Hainan Province, Hainan Institute for Tropical Agricultural Resources, Haikou, 571101, PR China. Electronic address: meiwenli@itbb.org.cn.

PMID: 37019169
DOI: 10.1016/j.phytochem.2023.113668

Abstract

Phytochemical investigation on the stems of Strophanthus divaricatus led to the isolation of four undescribed cardiac glycosides and one undescribed C₂₁ pregnane, together with eleven known steroids. Their structures were elucidated by a comprehensive analysis of HRESIMS, 1D and 2D NMR spectra. The absolute configuration of 16 was determined by comparison of the experimental and computed ECD spectra. Compounds 1-13 and 15 displayed potent to significant cytotoxicity against human cancer cell lines K562, SGC-7901, A549 and HeLa with IC₅₀ values of 0.02-16.08, 0.04-23.13, 0.06-22.31 and 0.06-15.13 μM, respectively.

Keywords: Apocynaceae; C(21) pregnane; Cardiac glycosides; Cytotoxicity; Strophanthus divaricatus.

MeSH terms

Antineoplastic Agents*
Cell Line, Tumor
Glycosides / chemistry
Humans
Molecular Structure
Pregnanes / chemistry
Pregnanes / pharmacology
Strophanthus*

Substances

Glycosides
Pregnanes
Antineoplastic Agents