Combined effects of intermittent fasting with swimming-based high intensity intermittent exercise training in Wistar rats

Everton Luis Dos Santos Cardoso; Fábio Cahuê; Iordan Emanuel Ferreira Miranda; Marcelo de Lima Sant'Anna; Cherley Borba Vieira Andrade; Raiana Andrade Quintanilha Barbosa; Tania Maria Ortiga-Carvalho; Mário Vaisman; Verônica Pinto Salerno

doi:10.1016/j.tice.2023.102099

Combined effects of intermittent fasting with swimming-based high intensity intermittent exercise training in Wistar rats

Tissue Cell. 2023 Jun:82:102099. doi: 10.1016/j.tice.2023.102099. Epub 2023 Apr 26.

Authors

Everton Luis Dos Santos Cardoso¹, Fábio Cahuê², Iordan Emanuel Ferreira Miranda³, Marcelo de Lima Sant'Anna⁴, Cherley Borba Vieira Andrade⁵, Raiana Andrade Quintanilha Barbosa⁶, Tania Maria Ortiga-Carvalho⁷, Mário Vaisman⁸, Verônica Pinto Salerno⁹

Affiliations

¹ Departamento de Biociência do Exercício, Escola de Educação Física e Desporto, Universidade Federal do Rio de Janeiro, (EEFD)/UFRJ, Brazil; Departamento de Endocrinologia Médica, Hospital Universitário Clementino Fraga Filho/(HUCFF)/UFRJ, Brazil.
² Departamento de Biociência do Exercício, Escola de Educação Física e Desporto, Universidade Federal do Rio de Janeiro, (EEFD)/UFRJ, Brazil; Instituto de Nutrição Josué de Castro, UFRJ, Brazil.
³ Departamento de Biociência do Exercício, Escola de Educação Física e Desporto, Universidade Federal do Rio de Janeiro, (EEFD)/UFRJ, Brazil; Instituto de Biofísica Carlos Chagas Filho, (IBCCF)/UFRJ, Brazil.
⁴ Centro de Instrução Almirante Sylvio de Camargo, Corpo de Fuzileiros Navais, Brazil.
⁵ Instituto de Biofísica Carlos Chagas Filho, (IBCCF)/UFRJ, Brazil; Departamento de Histologia e Embriologia, Universidade Estadual do Rio de Janeiro, (IBRAG)/UERJ, Brazil.
⁶ Instituto de Biofísica Carlos Chagas Filho, (IBCCF)/UFRJ, Brazil; Centro de Tecnologia Celular, Instituto Nacional de Cardiologia, RJ, Brazil.
⁷ Instituto de Biofísica Carlos Chagas Filho, (IBCCF)/UFRJ, Brazil.
⁸ Departamento de Endocrinologia Médica, Hospital Universitário Clementino Fraga Filho/(HUCFF)/UFRJ, Brazil.
⁹ Departamento de Biociência do Exercício, Escola de Educação Física e Desporto, Universidade Federal do Rio de Janeiro, (EEFD)/UFRJ, Brazil. Electronic address: vpsalerno@yahoo.com.br.

PMID: 37141748
DOI: 10.1016/j.tice.2023.102099

Abstract

High caloric intake and physical inactivity are known precursors to the development of several chronic metabolic diseases. For obesity and sedentarism, High Intensity Intermittent Exercise (HIIE) and Intermittent Fasting (IF) have emerged as individual strategies to attenuate their negative effects by improving metabolism. To study their combined effects, Wistar male rats (n = 74, 60 days old) were divided into four groups: Sedentary Control (C), swimming-based HIIE only (HIIE), Intermittent Fasting only (IF), and swimming-based HIIE associated with Intermittent Fasting (HIIE/IF). Over an eight-week period swimming performance, body composition, weight and feeding behavior were analyzed. The final morphology of white adipose tissue showed a significant reduction in adipocyte size consistent with a higher number of cells per area in exercised animals (vs C and IF, p < 0.05), which also displayed characteristics of browning through UCP-1 levels and CD31 staining. These results suggest that the increased performance in the HIIE/IF group is, in part, by modifications of WAT metabolism through the browning process.

Keywords: Adipose; High Intensity Intermittent Exercise; Intermittent Fasting; Obesity.

MeSH terms

Animals
Fasting
High-Intensity Interval Training* / methods
Intermittent Fasting
Male
Obesity
Rats
Rats, Wistar
Swimming*