IgG antibodies label the spermatozoan centriole of Drosophila melanogaster

MicroPubl Biol. 2023 May 26:2023:10.17912/micropub.biology.000838. doi: 10.17912/micropub.biology.000838. eCollection 2023.

Abstract

Centrioles are microtubule-based, barrel-shaped, subcellular organelles with evolutionarily conserved composition, structure, and function. Yet, in sperm cells, centrioles are remodeled, gaining species-specific composition and structure. The sperm centrioles of Drosophila melanogaster undergo dramatic remodeling, during which most known centriolar proteins are lost. Here, we find that IgG antibodies unexpectedly label Drosophila melanogaster spermatozoan centrioles . This labeling offers a simple method for marking the spermatozoan centriole, though it may interfere with testing new anti-centriolar antibodies using immunofluorescence.

Grants and funding

This project was supported by Agriculture and Food Research Initiative Competitive Grant no. OHOW-2020-02790 from the USDA National Institute of Food and Agriculture.