Identification of early gene expression profiles associated with long-lasting antibody responses to the Ebola vaccine Ad26.ZEBOV/MVA-BN-Filo

Fabiola Blengio; Hakim Hocini; Laura Richert; Cécile Lefebvre; Mélany Durand; Boris Hejblum; Pascaline Tisserand; Chelsea McLean; Kerstin Luhn; Rodolphe Thiebaut; Yves Levy

doi:10.1016/j.celrep.2023.113101

Identification of early gene expression profiles associated with long-lasting antibody responses to the Ebola vaccine Ad26.ZEBOV/MVA-BN-Filo

Cell Rep. 2023 Sep 26;42(9):113101. doi: 10.1016/j.celrep.2023.113101. Epub 2023 Sep 8.

Authors

Affiliations

¹ Vaccine Research Institute, Université Paris-Est Créteil, Faculté de Médecine, INSERM U955, Team 16, Créteil, France.
² Vaccine Research Institute, Université Paris-Est Créteil, Faculté de Médecine, INSERM U955, Team 16, Créteil, France; University Bordeaux, Department of Public Health, INSERM Bordeaux Population Health Research Centre, Inria SISTM, UMR 1219, Bordeaux, France; CHU de Bordeaux, Pôle de Santé Publique, Service d'Information Médicale, Bordeaux, France.
³ University Bordeaux, Department of Public Health, INSERM Bordeaux Population Health Research Centre, Inria SISTM, UMR 1219, Bordeaux, France; CHU de Bordeaux, Pôle de Santé Publique, Service d'Information Médicale, Bordeaux, France.
⁴ Janssen Vaccines & Prevention, B.V. Archimediesweg, Leiden, the Netherlands.
⁵ Vaccine Research Institute, Université Paris-Est Créteil, Faculté de Médecine, INSERM U955, Team 16, Créteil, France; University Bordeaux, Department of Public Health, INSERM Bordeaux Population Health Research Centre, Inria SISTM, UMR 1219, Bordeaux, France; CHU de Bordeaux, Pôle de Santé Publique, Service d'Information Médicale, Bordeaux, France. Electronic address: rodolphe.thiebaut@u-bordeaux.fr.
⁶ Vaccine Research Institute, Université Paris-Est Créteil, Faculté de Médecine, INSERM U955, Team 16, Créteil, France; Assistance Publique-Hôpitaux de Paris, Groupe Henri-Mondor Albert-Chenevier, Service Immunologie Clinique, Créteil, France. Electronic address: yves.levy@aphp.fr.

PMID: 37691146
DOI: 10.1016/j.celrep.2023.113101

Abstract

Ebola virus disease is a severe hemorrhagic fever with a high fatality rate. We investigate transcriptome profiles at 3 h, 1 day, and 7 days after vaccination with Ad26.ZEBOV and MVA-BN-Filo. 3 h after Ad26.ZEBOV injection, we observe an increase in genes related to antigen presentation, sensing, and T and B cell receptors. The highest response occurs 1 day after Ad26.ZEBOV injection, with an increase of the gene expression of interferon-induced antiviral molecules, monocyte activation, and sensing receptors. This response is regulated by the HESX1, ATF3, ANKRD22, and ETV7 transcription factors. A plasma cell signature is observed on day 7 post-Ad26.ZEBOV vaccination, with an increase of CD138, MZB1, CD38, CD79A, and immunoglobulin genes. We have identified early expressed genes correlated with the magnitude of the antibody response 21 days after the MVA-BN-Filo and 364 days after Ad26.ZEBOV vaccinations. Our results provide early gene signatures that correlate with vaccine-induced Ebola virus glycoprotein-specific antibodies.

Keywords: CP: Immunology; Ebola vaccine; emerging infections; innate and adaptive immunity; transcriptome.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Antibodies, Viral
Antibody Formation
Ebola Vaccines* / genetics
Ebolavirus*
Hemorrhagic Fever, Ebola*
Humans
Transcriptome / genetics
Vaccination
Vaccinia virus

Substances

Ebola Vaccines
smallpox and monkeypox vaccine modified vaccinia ankara-bavarian nordic
Antibodies, Viral