Distillers dried grains with soluble and enzyme inclusion in the diet effects broilers performance, intestinal health, and microbiota composition

Gabriela C Dal Pont; A Lee; C Bortoluzzi; N Rohloff Junior; Y Z Farnell; R Pilla; J S Suchodolski; M Ceccantini; C Eyng; M H Kogut

doi:10.1016/j.psj.2023.102981

Distillers dried grains with soluble and enzyme inclusion in the diet effects broilers performance, intestinal health, and microbiota composition

Poult Sci. 2023 Nov;102(11):102981. doi: 10.1016/j.psj.2023.102981. Epub 2023 Aug 8.

Authors

Gabriela C Dal Pont¹, A Lee¹, C Bortoluzzi¹, N Rohloff Junior², Y Z Farnell¹, R Pilla³, J S Suchodolski³, M Ceccantini⁴, C Eyng⁵, M H Kogut⁶

Affiliations

¹ Department of Poultry Science, Texas A&M AgriLife Research, Texas A&M University, College Station, TX 77843.
² Department of Animal Science, Western Paraná State University, Unioeste, Marechal C. Rondon, PR 85960000, Brazil.
³ Gastrointestinal Laboratory, Department of Small Animal Clinical Sciences, Texas A&M University, College Station, TX 77843.
⁴ Adisseo France S.A.S., 92160 Antony, France.
⁵ Department of Animal Science, Western Paraná State University, Unioeste, Marechal C. Rondon, PR 85960000, Brazil. Electronic address: cinthiaeyng@hotmail.com.
⁶ USDA-ARS, Southern Plains Agricultural Research Center, College Station, TX 77845.

Abstract

This study tested the effect of distillers dried grains with soluble (DDGS) inclusion in a broiler diet, with or without supplementation of exogenous enzymes, on the microbiota composition, intestinal health, diet digestibility and performance. A total of 288 one-day-old chickens was assigned to 6 treatments (8 replicate of 6 birds each) according to a completely randomized design with a 3 × 2 factorial scheme with 3 DDGS levels (0, 7 and 14%) and 2 inclusions of exogenous enzymes (with or without a multicarbohydrase complex + phytase [MCPC]). The results exhibited that DDGS inclusion up to 14% did not impair broilers performance up to 28 d, however, DDGS-fed animals exhibited significant improvement with the MCPC supplementation. No effects of the enzymes in the ileal digestibility were found at 21 d. DDGS inclusion in the diet affected dry matter and gross energy digestibility. Broilers fed diets with MCPC were found to have less intestinal histological alteration thus better gut health. No effect of DDGS, enzyme or interaction of those were observed for intestinal permeability and in the serum inflammatory biomarker (calprotectin) at 7 and 28 d. The increase of DDGS percentage in the diet reduced the diversity of the ileal microbiota but increased the cecal microbiota diversity. The inclusion of DDGS showed positive effects on microbiota composition due to a reduction of Proteobacteria phylum in the ileum at 28d and a reduction in the presence of Enterococcaceae family in the ileum at 14 and 28d. The inclusion of MCPC complex might promote beneficial changes in the ileal and cecal microbiota due reduce of Proteobacteria, Bacillaceae and Enterobacteriaceae. The supplementation of xylanase, β-glucanase, arabinofuranosidase and phytase to a DDGS diet improves performance and intestinal health allowing the use of these subproduct in the poultry nutrition.

Keywords: carbohydrase; corn subproduct; gut health; histological lesion; intestinal microbiota.