Impact of varying light spectral compositions on photosynthesis, morphology, chloroplast ultrastructure, and expression of light-responsive genes in Marchantia polymorpha

Pavel Pashkovskiy; Lyudmila Khalilova; Mikhail Vereshchagin; Alexander Voronkov; Tatiana Ivanova; Anatoliy A Kosobryukhov; Suleyman I Allakhverdiev; Vladimir D Kreslavski; Vladimir V Kuznetsov

doi:10.1016/j.plaphy.2023.108044

Impact of varying light spectral compositions on photosynthesis, morphology, chloroplast ultrastructure, and expression of light-responsive genes in Marchantia polymorpha

Plant Physiol Biochem. 2023 Oct:203:108044. doi: 10.1016/j.plaphy.2023.108044. Epub 2023 Sep 25.

Authors

Pavel Pashkovskiy¹, Lyudmila Khalilova², Mikhail Vereshchagin³, Alexander Voronkov⁴, Tatiana Ivanova⁵, Anatoliy A Kosobryukhov⁶, Suleyman I Allakhverdiev⁷, Vladimir D Kreslavski⁸, Vladimir V Kuznetsov⁹

Affiliations

¹ K.A. Timiryazev Institute of Plant Physiology, Russian Academy of Sciences, Botanicheskaya Street 35, Moscow, 127276, Russia. Electronic address: pashkovskiy.pavel@gmail.com.
² K.A. Timiryazev Institute of Plant Physiology, Russian Academy of Sciences, Botanicheskaya Street 35, Moscow, 127276, Russia. Electronic address: lhalilova@mail.ru.
³ K.A. Timiryazev Institute of Plant Physiology, Russian Academy of Sciences, Botanicheskaya Street 35, Moscow, 127276, Russia. Electronic address: mhlvrh@mail.ru.
⁴ K.A. Timiryazev Institute of Plant Physiology, Russian Academy of Sciences, Botanicheskaya Street 35, Moscow, 127276, Russia. Electronic address: voronkov_as@mail.ru.
⁵ K.A. Timiryazev Institute of Plant Physiology, Russian Academy of Sciences, Botanicheskaya Street 35, Moscow, 127276, Russia. Electronic address: itv_2006@mail.ru.
⁶ Institute of Basic Biological Problems, Russian Academy of Sciences, Institutskaya Street 2, Pushchino, Moscow Region, 142290, Russia. Electronic address: kosobr@rambler.ru.
⁷ K.A. Timiryazev Institute of Plant Physiology, Russian Academy of Sciences, Botanicheskaya Street 35, Moscow, 127276, Russia. Electronic address: suleyman.allakhverdiev@gmail.com.
⁸ Institute of Basic Biological Problems, Russian Academy of Sciences, Institutskaya Street 2, Pushchino, Moscow Region, 142290, Russia. Electronic address: vkreslav@rambler.ru.
⁹ K.A. Timiryazev Institute of Plant Physiology, Russian Academy of Sciences, Botanicheskaya Street 35, Moscow, 127276, Russia. Electronic address: vlkuzn@mail.ru.

PMID: 37776673
DOI: 10.1016/j.plaphy.2023.108044

Abstract

Marchantia polymorpha is a convenient model for studying light of different spectral compositions on various physiological and biochemical processes because its photoreceptor system is vastly simplified. The influence of red light (RL, 660 nm), far-red light (FRL, 730 nm), blue light (BL, 450 nm), and green light (GL, 525 nm) compared to white light (high-pressure sodium light (HPSL), white LEDs (WL 450 + 580 nm) and white fluorescent light (WFL) on photosynthetic and transpiration rates, photosystem II (PSII) activity, photomorphogenesis, and the expression of light and hormonal signaling genes was studied. The ultrastructure of the chloroplasts in different tissues of the gametophyte M. polymorpha was examined. FRL led to the formation of agranal chloroplasts (in the epidermis and the chlorenchyma) with a high starch content (in the parenchyma), which led to a reduced intensity of photosynthesis. BL increased the transcription of genes for the biosynthesis of secondary metabolites - chalcone synthase (CHS), cellulose synthase (CELL), and L-ascorbate peroxidase (APOX3), which is consistent with the increased activity of low-molecular weight antioxidants. FRL increased the expression of phytochrome apoprotein (PHY) and cytokinin oxidase (CYTox) genes, but the expression of the phytochrome interacting factor (PIF) gene decreased, which was accompanied by a significant change in gametophyte morphology. Analysis of crosstalk gene expression, and changes in morphology and photosynthetic activity was carried out.

Keywords: Blue light; Chloroplast ultrastructure; Far-red light; Green light; Light signaling genes; Morphology; Photomorphogenesis; Photosynthesis; Red light.