13C and 15N resonance assignments of alpha synuclein fibrils amplified from Lewy Body Dementia tissue

Alexander M Barclay; Dhruva D Dhavale; Collin G Borcik; Moses H Milchberg; Paul T Kotzbauer; Chad M Rienstra

doi:10.1007/s12104-023-10156-0

¹³C and ¹⁵N resonance assignments of alpha synuclein fibrils amplified from Lewy Body Dementia tissue

Biomol NMR Assign. 2023 Dec;17(2):281-286. doi: 10.1007/s12104-023-10156-0. Epub 2023 Nov 3.

Authors

Alexander M Barclay¹, Dhruva D Dhavale², Collin G Borcik^{3

4}, Moses H Milchberg^{3

5}, Paul T Kotzbauer⁶, Chad M Rienstra^{7

8

9

10}

Affiliations

¹ Center for Biophysics and Quantitative Biology, University of Illinois at Urbana-Champaign, Urbana, IL, 61801, USA.
² Department of Neurology, Washington University School of Medicine, St. Louis, MO, 63110, USA.
³ Department of Biochemistry, University of Wisconsin-Madison, Madison, WI, 53706, USA.
⁴ National Magnetic Resonance Facility at Madison, University of Wisconsin-Madison, Madison, WI, 53706, USA.
⁵ Graduate Program in Biophysics, University of Wisconsin-Madison, Madison, WI, 53706, USA.
⁶ Department of Neurology, Washington University School of Medicine, St. Louis, MO, 63110, USA. kotzbauerp@wustl.edu.
⁷ Department of Biochemistry, University of Wisconsin-Madison, Madison, WI, 53706, USA. crienstra@wisc.edu.
⁸ National Magnetic Resonance Facility at Madison, University of Wisconsin-Madison, Madison, WI, 53706, USA. crienstra@wisc.edu.
⁹ Graduate Program in Biophysics, University of Wisconsin-Madison, Madison, WI, 53706, USA. crienstra@wisc.edu.
¹⁰ Department of Chemistry, University of Illinois at Urbana-Champaign, Urbana, IL, 61801, USA. crienstra@wisc.edu.

Abstract

Fibrils of the protein α-synuclein (Asyn) are implicated in the pathogenesis of Parkinson Disease, Lewy Body Dementia, and Multiple System Atrophy. Numerous forms of Asyn fibrils have been studied by solid-state NMR and resonance assignments have been reported. Here, we report a new set of ¹³C, ¹⁵N assignments that are unique to fibrils obtained by amplification from postmortem brain tissue of a patient diagnosed with Lewy Body Dementia.

Keywords: Fibrils; Lewy Body Dementia; Parkinson Disease; Polymorphism; αSynuclein.

Publication types

Research Support, Non-U.S. Gov't
Research Support, N.I.H., Extramural

MeSH terms

Humans
Lewy Body Disease* / pathology
Multiple System Atrophy* / metabolism
Multiple System Atrophy* / pathology
Nuclear Magnetic Resonance, Biomolecular
Parkinson Disease* / metabolism
Parkinson Disease* / pathology
alpha-Synuclein / chemistry

Substances

alpha-Synuclein

Abstract

Publication types

MeSH terms

Substances

Grants and funding