Phenotypic Analysis of Early Neurogenesis in a Mouse Chimeric Embryo and Stem Cell-Based Neuruloid Model

Riley McMahon; V Pragathi Masamsetti; Patrick P L Tam

doi:10.1007/978-1-0716-3585-8_14

Phenotypic Analysis of Early Neurogenesis in a Mouse Chimeric Embryo and Stem Cell-Based Neuruloid Model

Methods Mol Biol. 2024:2746:165-177. doi: 10.1007/978-1-0716-3585-8_14.

Authors

Riley McMahon^{1

2}, V Pragathi Masamsetti^{1

2}, Patrick P L Tam^{3

4}

Affiliations

¹ Embryology Research Unit, Children's Medical Research Institute, University of Sydney, Westmead, NSW, Australia.
² School of Medical Sciences, Faculty of Medicine and Health, University of Sydney, Sydney, NSW, Australia.
³ Embryology Research Unit, Children's Medical Research Institute, University of Sydney, Westmead, NSW, Australia. ptam@cmri.org.au.
⁴ School of Medical Sciences, Faculty of Medicine and Health, University of Sydney, Sydney, NSW, Australia. ptam@cmri.org.au.

PMID: 38070089
DOI: 10.1007/978-1-0716-3585-8_14

Abstract

Analyzing the impact of genetic mutations on early neurogenesis of mammalian embryos in conventional mouse mutant models is laborious and time-consuming. To overcome these constraints and to fast-track the phenotypic analysis, we developed a protocol that harnesses the amenability of engineering genetic modifications in embryonic stem cells from which mid-gestation mouse chimeras and in vitro neuruloids are generated. These stem cell-based chimera and neuruloid experimental models allow phenotyping at early developmental time points of neurogenesis.

Keywords: Confocal microscopy; Embryos; Immunostaining; Neurogenesis; Stem cells.

MeSH terms

Animals
Embryo, Mammalian*
Embryonic Stem Cells*
Mammals
Mice
Neurogenesis / genetics