PANEL 3: Otitis media animal models, cell culture, tissue regeneration & pathophysiology

Arwa Kurabi; Kalyan Dewan; Joseph E Kerschner; Anke Leichtle; Jian-Dong Li; Peter Luke Santa Maria; Diego Preciado

doi:10.1016/j.ijporl.2023.111814

PANEL 3: Otitis media animal models, cell culture, tissue regeneration & pathophysiology

Int J Pediatr Otorhinolaryngol. 2024 Jan:176:111814. doi: 10.1016/j.ijporl.2023.111814. Epub 2023 Dec 10.

Authors

Arwa Kurabi¹, Kalyan Dewan², Joseph E Kerschner³, Anke Leichtle⁴, Jian-Dong Li⁵, Peter Luke Santa Maria⁶, Diego Preciado⁷

Affiliations

¹ Department of Otolaryngology, University of California San Diego, School of Medicine, La Jolla, CA, USA. Electronic address: akurabi@health.ucsd.edu.
² Department of Infectious Diseases, College of Veterinary Medicine, University of Georgia, Athens, GA, USA.
³ Department of Otolaryngology and Communication Sciences, Medical College of Wisconsin, Milwaukee, WI, USA.
⁴ Department of Otorhinolaryngology, University of Luebeck, Luebeck, Germany.
⁵ Center for Inflammation, Immunity and Infection, Institute for Biomedical Sciences, Georgia State University, Atlanta, GA, USA.
⁶ Department of Otolaryngology - Head & Neck Surgery, Stanford University, Stanford, CA, USA.
⁷ Children's National Hospital, Division of Pediatric Otolaryngology, Washington, DC, USA.

PMID: 38101097
DOI: 10.1016/j.ijporl.2023.111814

Abstract

Objective: To review and summarize recently published key articles on the topics of animal models, cell culture studies, tissue biomedical engineering and regeneration, and new models in relation to otitis media (OM).

Data source: Electronic databases: PubMed, National Library of Medicine, Ovid Medline.

Review methods: Key topics were assigned to the panel participants for identification and detailed evaluation. The PubMed reviews were focused on the period from June 2019 to June 2023, in any of the objective subject(s) or keywords listed above, noting the relevant references relating to these advances with a global overview and noting areas of recommendation(s). The final manuscript was prepared with input from all panel members.

Conclusions: In conclusion, ex vivo and in vivo OM research models have seen great advancements in the past 4 years. From the usage of novel genetic and molecular tools to the refinement of in vivo inducible and spontaneous mouse models, to the introduction of a wide array of reliable middle ear epithelium (MEE) cell culture systems, the next five years are likely to experience exponential growth in OM pathophysiology discoveries. Moreover, advances in these systems will predictably facilitate rapid means for novel molecular therapeutic studies.

Keywords: Animal models of OM; Chronic OM model; In vitro OM mode; OM cell culture; OM experimental models; OM inflammation; OM progression and pathophysiology; Otitis media (OM).

Publication types

Review

MeSH terms

Animals
Biomedical Engineering
Cell Culture Techniques
Disease Models, Animal
Ear, Middle
Humans
Mice
Otitis Media* / drug therapy