Icariin negatively regulated lipopolysaccharide-induced inflammation and ameliorated the odontogenic activity of human dental pulp cells in vitro

Guo Liu; Kkot-Byeol Bae; Ying Yang; Bin-Na Lee; Yun-Chan Hwang

doi:10.1016/j.heliyon.2023.e23282

Icariin negatively regulated lipopolysaccharide-induced inflammation and ameliorated the odontogenic activity of human dental pulp cells in vitro

Heliyon. 2023 Dec 3;9(12):e23282. doi: 10.1016/j.heliyon.2023.e23282. eCollection 2023 Dec.

Authors

Guo Liu^{1

2}, Kkot-Byeol Bae², Ying Yang³, Bin-Na Lee², Yun-Chan Hwang²

Affiliations

¹ Department of Endodontics, School and Hospital of Stomatology, Wenzhou Medical University, Wenzhou, 325000, China.
² Department of Conservative Dentistry, School of Dentistry, Dental Science Research Institute, Chonnam National University, Gwangju, 61186, South Korea.
³ Dental Implant Center, School and Hospital of Stomatology, Wenzhou Medical University, Wenzhou, 325000, China.

Abstract

Alleviating inflammation and promoting dentine regeneration is critical for the healing of pulpitis. In this study, we investigated the anti-inflammatory, angiogenesis and odontogenesis function of icariin on Human dental pulp cells (HDPCs) under inflammatory state. Furthermore, the underlying mechanisms was also evaluated. Icariin attenuated the LPS-induced pro-inflammatory marker expression, such as interleukin-1β (IL-1β), IL-6 and IL-8. The immunoblotting and immunofluorescence staining results showed that icariin suppressed the inflammatory responses mediated by the protein kinase B (Akt) and nuclear factor kappa-B (NF-κB) signaling cascades. Additionally, icariin also upregulated the expression of odontogenic and angiogenic genes and proteins (namely dentin sialophosphoprotein (DSPP), dentin matrix protein 1 (DMP1), anti-collagen Ⅰ (COL-Ⅰ), and vascular endothelial growth factor (VEGF) and fibroblast growth factor-1 (FGF-1)), alkaline phosphatase activity, and calcium nodule deposition in LPS-exposed HDPCs. In a word, our findings indicated that icariin attenuated pulp inflammation and promoted odontogenic and angiogenic differentiation in the inflammatory state. Icariin may be a promising vital pulp therapy agent for the regenerative treatment of the inflamed dental pulp.

Keywords: Angiogenesis; Anti-Inflammation; Icariin; Odontoblastic differentiation; Pulp regenerative treatment.