Single Cell Isolation from Surgically Resected Tissue Via Mechanical Dissociation Using TissueGrinder

Abstract

Primary cells form the basis of modern-day in vitro research analysis tools. Many conventional procedures for generating single-cell suspensions from solid tissue are neither robust nor reproducible. Here we describe primary cells isolation from surgically resected tumor tissue via enzyme-free mechanical dissociation using TissueGrinder, a novel semi-automated benchtop device. The isolated cells can be used for any downstream biochemical or cell-based analytic assay.

Keywords: Enzyme-free cell isolation; Mechanical dissociation; Primary cells; Single-cell isolation.