Effect of human umbilical cord blood-mesenchymal stem cells on cisplatin-induced nephrotoxicity in rats

Samia Hussein; Mai M Hasan; Abeer A Saeed; Asmaa M Tolba; Reham Sameh; Eman M A Abdelghany

doi:10.1007/s11033-023-08958-5

Effect of human umbilical cord blood-mesenchymal stem cells on cisplatin-induced nephrotoxicity in rats

Mol Biol Rep. 2024 Jan 28;51(1):234. doi: 10.1007/s11033-023-08958-5.

Authors

Samia Hussein¹, Mai M Hasan², Abeer A Saeed², Asmaa M Tolba³, Reham Sameh⁴, Eman M A Abdelghany³

Affiliations

¹ Medical Biochemistry and Molecular Biology Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt. samiahussein82@hotmail.com.
² Physiology Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt.
³ Anatomy and Embryology Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt.
⁴ Pathology Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt.

PMID: 38282086
DOI: 10.1007/s11033-023-08958-5

Abstract

Background: Cisplatin-containing regimen is an effective treatment for several malignancies. However, cisplatin is an important cause of nephrotoxicity. So, many trials were performed to transplant stem cells systemically or locally to control cisplatin-induced nephrotoxicity. Stem cell therapeutic effect may be dependent on the regulation of inflammation and oxidant stress.

Aim: To investigate the effect of human umbilical cord blood-mesenchymal stem cells (hUCB-MSCs) on the histological structure, the oxidant stress, and the inflammatory gene expression in an experimental model of cisplatin-induced nephrotoxicity in rats.

Method: The rats were divided into 6 equal groups (each of 10 rats): Group I included normal rats that received no treatment. Group II included healthy rats that received IV hUCB-MSCs. Group III included untreated cisplatin-induced nephrotoxic rats. Group IV included cisplatin-induced nephrotoxic rats that received magnesium (Mg) injections after injury. Group V was injected with hUCB-MSCs after injury. Group VI received both Mg and hUCB-MSCs after injury. In tissue homogenates, reduced glutathione (GSH), superoxide dismutase (SOD), and malondialdehyde (MDA) activities were measured. Quantitative real-time-polymerase chain reaction (qRT-PCR) was performed to assess iNOS, TLR4, and NF-kB gene expression. Hematoxylin and eosin (H&E) staining was performed to study the histological structure of the kidney. Immunohistochemical staining of iNOS and NF-κB was performed, as well.

Results: Disturbed kidney functions, oxidative status, and histological structure were seen in the rats that received cisplatin. Treated groups showed improvements in kidney functions, oxidative status, and histological structure, particularly in the combined treatment group.

Conclusion: In the cisplatin-induced nephrotoxicity model, hUCB-MSCs could improve the functional and morphological kidney structure by modulation of oxidative and inflammatory status.

Keywords: Cisplatin; Human umbilical cord blood-mesenchymal stem cells; Mg; Nephrotoxicity; qRT- PCR.

MeSH terms

Animals
Cisplatin / adverse effects
Cisplatin / metabolism
Fetal Blood
Humans
Mesenchymal Stem Cell Transplantation*
Mesenchymal Stem Cells* / metabolism
Oxidants / metabolism
Rats
Stem Cells

Substances

Cisplatin
Oxidants