Production of Influenza Virus Glycoproteins Using Insect Cells

Madhumathi Loganathan; Benjamin Francis; Florian Krammer

doi:10.1007/978-1-0716-3666-4_4

Production of Influenza Virus Glycoproteins Using Insect Cells

Methods Mol Biol. 2024:2762:43-70. doi: 10.1007/978-1-0716-3666-4_4.

Authors

Madhumathi Loganathan^{1

2}, Benjamin Francis^{1

2}, Florian Krammer^{3

4

5}

Affiliations

¹ Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, NY, USA.
² Center for Vaccine Research and Pandemic Preparedness (C-VaRPP), Icahn School of Medicine at Mount Sinai, New York, NY, USA.
³ Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, NY, USA. florian.krammer@mssm.edu.
⁴ Center for Vaccine Research and Pandemic Preparedness (C-VaRPP), Icahn School of Medicine at Mount Sinai, New York, NY, USA. florian.krammer@mssm.edu.
⁵ Department of Pathology, Molecular and Cell Based Medicine, Icahn School of Medicine at Mount Sinai, New York, NY, USA. florian.krammer@mssm.edu.

PMID: 38315359
DOI: 10.1007/978-1-0716-3666-4_4

Abstract

The baculovirus/insect cell expression system is a very useful tool for reagent and antigen generation in vaccinology, virology, and immunology. It allows for the production of recombinant glycoproteins, which are used as antigens in vaccination studies and as reagents in immunological assays. Here, we describe the process of recombinant glycoprotein production using the baculovirus/insect cell expression system.

Keywords: Baculovirus; Hemagglutinin; Influenza virus; Insect cells; Neuraminidase; Protein production; Viral glycoproteins.

MeSH terms

Animals
Baculoviridae / genetics
Baculoviridae / metabolism
Glycoproteins / genetics
Glycoproteins / metabolism
Hemagglutinin Glycoproteins, Influenza Virus
Influenza A Virus, H5N1 Subtype*
Influenza Vaccines*
Insecta / metabolism
Recombinant Proteins

Substances

Glycoproteins
Hemagglutinin Glycoproteins, Influenza Virus
Recombinant Proteins
Influenza Vaccines