The potential impact of polymorphisms in METTL3 gene on knee osteoarthritis susceptibility

Houlin Mi; Mingzhi Wang; Yongmei Chang

doi:10.1016/j.heliyon.2024.e28035

The potential impact of polymorphisms in METTL3 gene on knee osteoarthritis susceptibility

Heliyon. 2024 Mar 19;10(7):e28035. doi: 10.1016/j.heliyon.2024.e28035. eCollection 2024 Apr 15.

Authors

Houlin Mi¹, Mingzhi Wang², Yongmei Chang³

Affiliations

¹ Department of Orthopedics, South China Hospital Affiliated to Shenzhen University, 1# Fuxin Road, Longgang District, Shenzhen City, Guangdong Province, 518111, China.
² Department of Thoracic Surgery, Guangdong Second Provincial General Hospital, 466# Xingang Middle Road, Haizhu District, Guangzhou City, Guangdong Province, 510006, China.
³ Department of Respiratory Medicine, Guangdong Second Provincial General Hospital, 466# Xingang Middle Road, Haizhu District, Guangzhou City, Guangdong Province, 510006, China.

Abstract

Objective: This study was aimed to explore the correlation between METTL3 polymorphisms and susceptibility to knee osteoarthritis (KOA).

Methods: The relationship of five single nucleotide polymorphisms (SNPs) in the METTL3 gene with the susceptibility of KOA was analyzed through multinomial logistic regression analysis in this a case-control study. Genotyping was performed on 228 KOA patients and 252 unaffected individuals from South China based on the TaqMan method. The MDR software (version 3.0.2) was utilized for the analysis of SNP interactions.

Results: Out of the five SNPs examined, the T > G change in the METTL3 gene at the rs1061026 locus increased the risk of KOA, while rs1139130 A > G and rs1263802 C > T variants were found to be linked with a reduced risk of developing KOA with statistical significance. The rs1061027 A > C and rs1263801 C > G variants did not show significant association (p＞0.05). The rs1061026 TG/GG genotype showed a significant correlation with an increased risk of KOA in the following subgroups: the males, individuals with a BMI ranging from 24 to 28, smokers, those who were not engaged in physical exercise (PE), patients who had experienced KOA symptoms for eight years or longer, and those without a family history of the disease or reported swelling. On the other hand, the rs1139130 AG/GG genotype demonstrated a protective effect against KOA among the females, individuals with a BMI greater than or equal to 24, a unilateral KOA, or a KOA duration of 8 years or less, non-smokers, non-alcohol drinkers, those who were not engaged in PE, and those who had no injury or family history, or no experience of knee swelling. Additionally, it was observed that the rs1263802 CT/TT genotypes showed a protective effect among patients without a history of injury. Furthermore, individuals with the haplotypes GAT, GGC, TAT, and TGC were found to have a significantly lower susceptibility to KOA compared to the reference haplotype TAC.

Conclusions: The METTL3 gene variant rs1061026 could increase the risk of KOA, whereas the variants of rs1139130 as well as rs1263802 might exert a protective effect against KOA. These variants could potentially function as susceptibility markers for KOA among the population from South China.

Keywords: Knee osteoarthritis; METTL3; Polymorphism; Susceptibility.