Effect of dietary chitosan on the growth performance, intestinal histology and growth-related gene expression in stellate sturgeon (Acipenser stellatus) juveniles

Donya Zakeri; Jamileh Pazooki; Mahmoud Mohseni; Shirin Jamshidi

doi:10.1111/jpn.13961

Effect of dietary chitosan on the growth performance, intestinal histology and growth-related gene expression in stellate sturgeon (Acipenser stellatus) juveniles

J Anim Physiol Anim Nutr (Berl). 2024 Apr 11. doi: 10.1111/jpn.13961. Online ahead of print.

Authors

Donya Zakeri¹, Jamileh Pazooki¹, Mahmoud Mohseni², Shirin Jamshidi²

Affiliations

¹ Department of Animal Sciences and Marine Biology, Faculty of Life Sciences and Biotechnology, Shahid Beheshti University, Tehran, Iran.
² International Sturgeon Research Institute, Iranian Fisheries Science Research Institute, Agricultural Research Education and Extension Organization (AREEO), Rasht, Iran.

PMID: 38602249
DOI: 10.1111/jpn.13961

Abstract

As sturgeon breeding has proliferated, there has been a heightened demand for growth stimulators in their diets. This study aimed to determine the impact of dietary chitosan on growth performance, whole-body proximate composition, growth-related gene expression, and intestinal histology in juvenile Acipenser stellatus. A total of 180 A. stellatus juveniles with an average weight of 31.90 ± 0.73 g were fed with diets containing 0 (control), 1.5, 3.0, 4.5, and 6.0 g chitosan.kg^-1 basic diet for eight weeks. The findings revealed a significant enhancement in growth performance with rising chitosan concentrations. Furthermore, chitosan supplementation upregulated the expression of the growth hormone gene in both brain and liver tissues. In liver samples, the most pronounced expression of the insulin-like growth factor-1 gene was noted at 6.0 g chitosan.kg^-1, while in brain samples, peak expressions were observed in both the 4.5 and 6.0 g chitosan.kg^-1 treatments. While the whole-body proximate composition remained relatively stable, there was a notable decrease in whole-body lipids with the escalation of chitosan dosage. Intestinal villi dimensions, both height and width, were amplified in the chitosan-supplemented groups compared to controls. In summation, chitosan supplementation showed promise in bolstering growth performance, refining intestinal morphology, and enhancing growth-related gene expression. Analysis of the polynomial regression of weight gain and specific growth rate revealed that the optimum dietary chitosan requirements in A. stellatus were 5.32 and 5.21 g chitosan.kg^-1, respectively.

Keywords: aquaculture; chitin; feed additive; molecular analysis; real‐time pcr; starry sturgeon.