Next-generation sequencing survey of acute febrile illness in Senegal (2020-2022)

Gregory S Orf; Ambroise D Ahouidi; Maximillian Mata; Cyrille Diedhiou; Aminata Mboup; Abdou Padane; Noel Magloire Manga; Agbogbenkou Tevi Dela-Del Lawson; Francisco Averhoff; Michael G Berg; Gavin A Cloherty; Souleymane Mboup

doi:10.3389/fmicb.2024.1362714

Next-generation sequencing survey of acute febrile illness in Senegal (2020-2022)

Front Microbiol. 2024 Apr 9:15:1362714. doi: 10.3389/fmicb.2024.1362714. eCollection 2024.

Authors

Gregory S Orf^#^{1

2}, Ambroise D Ahouidi^#^{2

3}, Maximillian Mata^{1

2}, Cyrille Diedhiou^{2

3}, Aminata Mboup^{2

3}, Abdou Padane^{2

3}, Noel Magloire Manga⁴, Agbogbenkou Tevi Dela-Del Lawson⁵, Francisco Averhoff^{1

2}, Michael G Berg^{1

2}, Gavin A Cloherty^{1

2}, Souleymane Mboup^{2

3}

Affiliations

¹ Core Diagnostics, Abbott Laboratories, Abbott Park, IL, United States.
² Abbott Pandemic Defense Coalition, Abbott Park, IL, United States.
³ Institut de Recherche en Santé, de Surveillance Epidémiologique et de Formation, Dakar, Senegal.
⁴ Unit of Infectious and Tropical Diseases, Université Assane Seck, Hôpital de la Paix, Ziguinchor, Senegal.
⁵ Unit of Infectious and Tropical Diseases, Hôpital Mame Abdou Aziz Sy Dabakh, Tivaouane, Senegal.

^# Contributed equally.

Abstract

Introduction: Acute febrile illnesses (AFI) in developing tropical and sub-tropical nations are challenging to diagnose due to the numerous causes and non-specific symptoms. The proliferation of rapid diagnostic testing and successful control campaigns against malaria have revealed that non-Plasmodium pathogens still contribute significantly to AFI burden. Thus, a more complete understanding of local trends and potential causes is important for selecting the correct treatment course, which in turn will reduce morbidity and mortality. Next-generation sequencing (NGS) in a laboratory setting can be used to identify known and novel pathogens in individuals with AFI.

Methods: In this study, plasma was collected from 228 febrile patients tested negative for malaria at clinics across Senegal from 2020-2022. Total nucleic acids were extracted and converted to metagenomic NGS libraries. To identify viral pathogens, especially those present at low concentration, an aliquot of each library was processed with a viral enrichment panel and sequenced. Corresponding metagenomic libraries were also sequenced to identify non-viral pathogens.

Results and discussion: Sequencing reads for pathogens with a possible link to febrile illness were identified in 51/228 specimens, including (but not limited to): Borrelia crocidurae (N = 7), West Nile virus (N = 3), Rickettsia felis (N = 2), Bartonella quintana (N = 1), human herpesvirus 8 (N = 1), and Saffold virus (N = 1). Reads corresponding to Plasmodium falciparum were detected in 19 specimens, though their presence in the cohort was likely due to user error of rapid diagnostic testing or incorrect specimen segregation at the clinics. Mosquito-borne pathogens were typically detected just after the conclusion of the rainy season, while tick-borne pathogens were mostly detected before the rainy season. The three West Nile virus strains were phylogenetically characterized and shown to be related to both European and North American clades. Surveys such as this will increase the understanding of the potential causes of non-malarial AFI, which may help inform diagnostic and treatment options for clinicians who provide care to patients in Senegal.

Keywords: Senegal; acute febrile illness; malaria; next-generation sequencing; viral enrichment.

Grants and funding

The author(s) declare financial support was received for the research, authorship, and/or publication of the article. Funding was provided by Abbott Laboratories. The funder provided support in the form of salaries for the authors employed by the Abbott Laboratories but did not have any additional role in the study design, data collection and analysis, decision to publish, or preparation of the manuscript.