Heavy metals are found naturally; however, anthropogenic activities such as mining, inappropriate disposal of industrial waste, and the use of pesticides and fertilizers containing heavy metals can cause their unwanted release into the environment. Conventionally, detection of heavy metals is performed using atomic absorption spectrometry, electrochemical methods and inductively coupled plasma-mass spectrometry; however, they involve expensive and sophisticated instruments and multistep sample preparation that require expertise for accurate results. In contrast, microfluidic devices involve rapid, cost-efficient, simple, and reliable approaches for in-laboratory and real-time monitoring of heavy metals. The use of inexpensive and environment friendly materials for fabrication of microfluidic devices has increased the manufacturing efficiency of the devices. Different types of techniques used in heavy metal detection include colorimetry, absorbance-based, and electrochemical detection. This review provides insight into the detection of toxic heavy metals such as mercury (Hg), cadmium (Cd), lead (Pb), and arsenic (As). Importance is given to colorimetry, optical, and electrochemical techniques applied for the detection of heavy metals using microfluidics and their modifications to improve the limit of detection (LOD).