Evaluation of cytocompatibility and cell proliferation of electrospun chitosan/polyvinyl alcohol/montmorillonite clay scaffold with l929 cell lines in skin regeneration activity and in silico molecular docking studies

K Sangeetha; Mohammed F Albeshr; K Shoba; G Lavanya; P Supriya Prasad; P N Sudha

doi:10.1016/j.ijbiomac.2024.131762

Evaluation of cytocompatibility and cell proliferation of electrospun chitosan/polyvinyl alcohol/montmorillonite clay scaffold with l929 cell lines in skin regeneration activity and in silico molecular docking studies

Int J Biol Macromol. 2024 Apr 22;268(Pt 2):131762. doi: 10.1016/j.ijbiomac.2024.131762. Online ahead of print.

Authors

K Sangeetha¹, Mohammed F Albeshr², K Shoba³, G Lavanya⁴, P Supriya Prasad⁵, P N Sudha⁶

Affiliations

¹ Biomaterials Research Lab, Department of Chemistry, DKM College for Women (Autonomous), Vellore, Tamilnadu, India.
² Department of Zoology, College of Sciences, King Saud University, P.O. Box. 2455, Riyadh 11451, Saudi Arabia.
³ Department of Biochemistry, DKM College for Women (Autonomous), Vellore, Tamilnadu, India.
⁴ Department of Chemistry, Saveetha Engineering College, Thandalam, Chennai, Tamilnadu, India.
⁵ Department of Chemistry, Muthurangam Govt. Arts College, Vellore, Tamilnadu, India.
⁶ Biomaterials Research Lab, Department of Chemistry, DKM College for Women (Autonomous), Vellore, Tamilnadu, India; Department of Physiology, Saveetha Dental College & Hospitals, Saveetha Institute of Medical and Technical Sciences (SIMATS), Saveetha University, Chennai, Tamil Nadu, India. Electronic address: drparsu8@gmail.com.

PMID: 38657925
DOI: 10.1016/j.ijbiomac.2024.131762

Abstract

The present investigation describes the development of a novel Chitosan/Polyvinyl Alcohol/Montmorillonite Clay (CS/PVA/MMT) scaffold by adopting an electrospinning method, and their biocompatibility was evaluated in vitro with L929 fibroblast cell line to ascertain its use in wound healing applications. The fabricated scaffold was characterized using analytical techniques. FT-IR measurement exhibited the existence of relevant functional groups and XRD implies scaffolds' amorphous nature. The scaffold's morphology and pore diameter were assessed using TEM and SEM. The pore diameter of the as-prepared scaffold was approximately 125 nm. The antimicrobial assay of the scaffold was evaluated against selected pathogens which demonstrated higher antimicrobial efficacy. The scavenging activity tested using the DPPH assay showed remarkable scavenging capability. The wound healing properties were tested through the Cytotoxicity assay conducted on the L929 assay which proved the scaffold to be a suitable material for cell proliferation. Also, a Molecular docking investigation was carried out for CS/PVA/MMT ligand using human neutrophil elastase (HNE) 1H1B protein as a receptor in the CB-Dock server. Studies conducted in silico revealed strong interaction and high binding energy ratings of CS/PVA/MMT ligand with key residues of human neutrophil elastase (HNE) 1H1B proteins that help in tissue regeneration activity.

Keywords: Chitosan-based mat; In vitro L929 cell line; Molecular docking.