Protocol for enrichment and functional analysis of human hematopoietic cells from umbilical cord blood

Sarah Gutch; Lindsay Beasley; Scott Cooper; Mark H Kaplan; Maegan L Capitano; James Ropa

doi:10.1016/j.xpro.2024.103024

Protocol for enrichment and functional analysis of human hematopoietic cells from umbilical cord blood

STAR Protoc. 2024 Apr 23;5(2):103024. doi: 10.1016/j.xpro.2024.103024. Online ahead of print.

Authors

Sarah Gutch¹, Lindsay Beasley¹, Scott Cooper¹, Mark H Kaplan¹, Maegan L Capitano¹, James Ropa²

Affiliations

¹ Indiana University School of Medicine, Indianapolis, IN 46202, USA.
² Indiana University School of Medicine, Indianapolis, IN 46202, USA. Electronic address: jropa@iu.edu.

Abstract

Umbilical cord blood (CB) is a donor source for hematopoietic cell therapies. Understanding what drives hematopoietic stem and progenitor cell function is critical to our understanding of the usage of CB in hematopoietic cell therapies. Here, we describe how to isolate and analyze the function of human hematopoietic cells from umbilical CB. This protocol demonstrates assays that measure phenotypic properties and hematopoietic cell potency. For complete details on the use and execution of this protocol, please refer to Broxmeyer et al.¹.

Keywords: Cell Differentiation; Cell culture; Cell isolation; Stem Cells.