Background: The process of wound healing is complex, and expediting it remains a challenge. The advantages of extremely low frequency electric and magnetic fields (ELF-EMF) are its non-invasive treatment, promotes healing and promotes myogenesis of C2C12 cells. Epidermal growth factor (EGF) is known to play a vital role in promoting wound healing, so a combination of ELF-EMF and EGF can have far-reaching significance.
Objective: To study the effect of recombinant murine epidermal growth factor (rmEGF) combined with ELF-EMF on wound healing.
Methods: Thirty-six rats were randomly divided into three groups: normal control group, EGF group, and ELF-EMF+EGF group, and a 20 mm × 20 mm dorsal wound was made. The wound healing rate of rats was calculated on the 3rd, 7th, 11th and 15th day. HE staining was used to observe the micro-morphological changes during the wound healing process.
Results: The wound healing rate of EGF+ELF-EMF group was better than other groups. On the 15th day of wound healing, the wounds of each group were completely healed. On the 3rd, 7th, 11th and 15th day of HE staining, the early inflammatory cell infiltration, the arrangement of fibroblasts and the number of new capillaries in the wounds of EGF+ELF-EMF group were better than those of the other groups.
Conclusions: rmEGF combined with ELF-EMF significantly promotes wound healing in SD rats.
Keywords: EGF; EGFR; ELF-EMF; Extremely low frequency electromagnetic field; SD rats; epidermal growth factor; epidermal growth factor receptor; recombinant murine epidermal growth factor; rmEGF; sprague-dawley rats.