A synergistically antimicrobial and antioxidant hyaluronic acid hydrogel for infected wounds

Yongping Lu; Weiqi Kang; Yue Yu; Haiying Lu; Yuemin Wang; Zhe Xu; Jia Zeng; Meng Qin; Xinyuan Xu

doi:10.1016/j.ijbiomac.2024.131795

A synergistically antimicrobial and antioxidant hyaluronic acid hydrogel for infected wounds

Int J Biol Macromol. 2024 Apr 24;269(Pt 2):131795. doi: 10.1016/j.ijbiomac.2024.131795. Online ahead of print.

Authors

Yongping Lu¹, Weiqi Kang², Yue Yu², Haiying Lu², Yuemin Wang³, Zhe Xu², Jia Zeng², Meng Qin⁴, Xinyuan Xu⁵

Affiliations

¹ College of Polymer Science and Engineering, State Key Laboratory of Polymer Materials Engineering, Sichuan University, Chengdu 610065, PR China; Guangyuan Central Hospital, Guangyuan 628000, PR China.
² Guangyuan Central Hospital, Guangyuan 628000, PR China.
³ College of Polymer Science and Engineering, State Key Laboratory of Polymer Materials Engineering, Sichuan University, Chengdu 610065, PR China.
⁴ College of Polymer Science and Engineering, State Key Laboratory of Polymer Materials Engineering, Sichuan University, Chengdu 610065, PR China. Electronic address: qinmeng@scu.edu.cn.
⁵ College of Polymer Science and Engineering, State Key Laboratory of Polymer Materials Engineering, Sichuan University, Chengdu 610065, PR China. Electronic address: xinyuanxu22@scu.edu.cn.

PMID: 38670175
DOI: 10.1016/j.ijbiomac.2024.131795

Abstract

Bacterial infections during wound healing impede the healing process and trigger local or systemic inflammatory reactions. Consequently, there is an urgent need to develop a new material with antimicrobial and antioxidant properties to promote infected wound healing. A synergistically antimicrobial and antioxidant hyaluronic acid hydrogel (HMn) is prepared by employing MnO₂ nanosheets into 4ARM-PEG5000-SH crosslinked methacrylated hyaluronic acid (HAMA) network. The coordination between sulfhydryl groups of 4ARM-PEG5000-SH and MnO₂ nanosheets ensures entrapment of the nanosheets within the hydrogel, while the interaction between 4ARM-PEG5000-SH and HAMA results in facile gelation through thiol-ene click reaction. MnO₂ nanosheets exhibit strong photothermal properties and reactive oxygen species (ROS) scavenging abilities, while hyaluronic acid promotes wound healing. When subjected to near-infrared (NIR) irradiation, the HMn achieves a bactericidal rate of 95.24 % for Staphylococcus aureus and nearly 100 % for Escherichia coli. In animal experiments, treatment with the HMn under NIR irradiation results in the best wound healing outcomes. Both in vitro and vivo biocompatible assays demonstrate that the HMn has rarely cell cytotoxicity and tissue damage. The HMn is easy to prepare and has good biocompatibility as well as efficient antibacterial and antioxidant properties, providing a novel method for the treatment of infected wounds.

Keywords: Antibacterial; Antioxidant; Hydrogel; Photothermal therapy; Wound repair.