Role of ADAR1 on Proliferation and Differentiation in Porcine Preadipocytes

Menghuan Yang; Jun Jiang; Ruimin Ren; Ning Gao; Jun He; Yuebo Zhang

doi:10.3390/ani14081201

Role of ADAR1 on Proliferation and Differentiation in Porcine Preadipocytes

Animals (Basel). 2024 Apr 17;14(8):1201. doi: 10.3390/ani14081201.

Authors

Menghuan Yang^{1

2}, Jun Jiang^{1

2}, Ruimin Ren^{1

2}, Ning Gao^{1

2}, Jun He^{1

2}, Yuebo Zhang^{1

2}

Affiliations

¹ College of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, China.
² Key Laboratory of Livestock and Poultry Resources (Pig) Evaluation and Utilization, Ministry of Agriculture and Rural Affairs, Changsha 410128, China.

Abstract

Recent research has identified ADAR1 as a participant in the regulation of lipid accumulation in mice. However, there are no reports on the roles of ADAR1 in proliferation, apoptosis and differentiation of porcine preadipocytes. In this study, we investigated the role of ADAR1 in differentiation, proliferation and apoptosis of porcine preadipocytes using CCK-8, EdU staining, cell cycle detection, RT-qPCR, Western blot, a triglyceride assay and Oil Red O staining. The over-expression of ADAR1 significantly promoted proliferation but inhibited the differentiation and apoptosis of porcine preadipocytes. The inhibition of ADAR1 had the opposite effect on the proliferation, differentiation and apoptosis of porcine preadipocytes with over-expressed ADAR1. Then, the regulation mechanisms of ADAR1 on preadipocyte proliferation were identified using RNA-seq, and 197 DEGs in response to ADAR1 knockdown were identified. The MAPK signaling pathway is significantly enriched, indicating its importance in mediating fat accumulation regulated by ADAR1. The study's findings will aid in uncovering the mechanisms that regulate fat accumulation through ADAR1.

Keywords: ADAR1; RNA-seq; differentiation; preadipocyte; proliferation.

Grants and funding

This research was funded by the Excellent Youth Project of Hunan Provincial Department of Education (22B0209), Special Fund for the Construction of Innovative Provinces in Hunan (2021NK1009) and Hunan Province Science and Technology Talent Lifting Project (2022TJ-Q15).