A Novel NIR Fluorescence Probe with AIE Property to Image Viscosity in Nystatin-Induced Cell Model

Xiaokai Wang; Zhan Yin; Haoran Liu; Ziming Wang; Xiaofei Zhu; Yong Ye

doi:10.1007/s10895-024-03706-9

A Novel NIR Fluorescence Probe with AIE Property to Image Viscosity in Nystatin-Induced Cell Model

J Fluoresc. 2024 Apr 27. doi: 10.1007/s10895-024-03706-9. Online ahead of print.

Authors

Xiaokai Wang¹, Zhan Yin¹, Haoran Liu¹, Ziming Wang¹, Xiaofei Zhu², Yong Ye³

Affiliations

¹ Green Catalysis Center, College of Chemistry, Zhengzhou University, Zhengzhou, 450001, China.
² Green Catalysis Center, College of Chemistry, Zhengzhou University, Zhengzhou, 450001, China. Zhuxiaofei@zzu.edu.cn.
³ Green Catalysis Center, College of Chemistry, Zhengzhou University, Zhengzhou, 450001, China. yeyong03@tsinghua.org.cn.

PMID: 38676771
DOI: 10.1007/s10895-024-03706-9

Abstract

As one of the most significant parameters in cellular microenvironment, viscosity levels could be used to determine the metabolic process of bioactive substances within cells. Abnormal viscosity levels are closely associated with a series of diseases. Therefore, the design and synthesis of fluorescent probes that can monitor changes of intracellular viscosity in real-time is of great significance for the study of disease development process. Here, a new viscosity-recognized NIR fluorescence probe W1 based on quinoline-malonitrile is synthesized, and it is not susceptible to interference substances. Besides, AIE probe W1 shows fast response, excellent photostability, low cytotoxicity, good linear relationship between fluorescence intensity value and viscosity. Based on the above advantages, probe W1 is used to image the change of viscosity level in the cell model induced by nystatin.

Keywords: AIE; Fluorescence probe; Image; NIR; Viscosity.

Grants and funding

21907025/National Natural Science Foundation of China