Chip-based automated equipment for dual-mode point-of-care testing foodborne pathogens

Binfeng Yin; Haoyu Zhu; Shiyu Zeng; A S M Muhtasim Fuad Sohan; Xinhua Wan; Jun Liu; Pan Zhang; Xiaodong Lin

doi:10.1016/j.bios.2024.116338

Chip-based automated equipment for dual-mode point-of-care testing foodborne pathogens

Biosens Bioelectron. 2024 Aug 1:257:116338. doi: 10.1016/j.bios.2024.116338. Epub 2024 Apr 25.

Authors

Binfeng Yin¹, Haoyu Zhu², Shiyu Zeng², A S M Muhtasim Fuad Sohan³, Xinhua Wan², Jun Liu⁴, Pan Zhang⁵, Xiaodong Lin⁶

Affiliations

¹ School of Mechanical Engineering, Yangzhou University, Yangzhou, 225127, China. Electronic address: binfengyin@yzu.edu.cn.
² School of Mechanical Engineering, Yangzhou University, Yangzhou, 225127, China.
³ School of Electrical and Mechanical Engineering, The University of Adelaide, Adelaide, SA 5005, Australia.
⁴ Suqian Product Quality Supervision and Inspection Institute, Suqian, 223800, China.
⁵ National Key Lab of MicroNanofabrication Technology, School of Integrated Circuits, Peking University, Beijing, 100871, China. Electronic address: panzhangpku@gmail.com.
⁶ University of Macau Zhuhai UM Science and Technology Research Institute, Zhuhai, 519000, China. Electronic address: lxdlxf2011@163.com.

PMID: 38677017
DOI: 10.1016/j.bios.2024.116338

Abstract

Foodborne pathogens have a substantial bearing on food safety and environmental health. The development of automated, portable and compact devices is essential for the on-site and rapid point-of-care testing (POCT) of bacteria. Here, this work developed a micro-automated microfluidic device for detecting bacteria, such as Escherichia coli (E. coli) O157:H7, using a seashell-like microfluidic chip (SMC) as an analysis and mixing platform. The automated device integrates a colorimetric/fluorescent system for the metabolism of copper (Cu²⁺) by E. coli affecting o-phenylenediamine (OPD) for concentration analysis. A smartphone was used to read the RGB data of the chip reaction reservoir to detect colorimetric and fluorescence patterns in the concentration range of 10²-10⁶ CFU mL^-1. The automated device overcomes the low efficiency and tedious steps of traditional detection and enables high-precision automated detection that can be applied to POCT in the field, providing an ideal solution for broadening the application of E. coli detection.

Keywords: Dual-mode detection; Escherichia coli; Micro-automated device; Microfluidic chip; Point-of-care testing (POCT).

MeSH terms

Biosensing Techniques* / instrumentation
Biosensing Techniques* / methods
Colorimetry* / instrumentation
Copper* / chemistry
Equipment Design*
Escherichia coli Infections / microbiology
Escherichia coli O157* / isolation & purification
Food Contamination / analysis
Food Microbiology*
Foodborne Diseases / microbiology
Humans
Lab-On-A-Chip Devices*
Phenylenediamines / chemistry
Point-of-Care Testing*
Smartphone / instrumentation

Substances

Copper
Phenylenediamines